Methods of expanding FCs or stem cells are provided herein, as are methods of screening for compounds that increase expression of DOCK2 or decrease expression of Arhgap18, either of which improves engraftment.

Provided are a culture vessel for NK cells and a method for culture of NK cells, which can provide high NK cell culture efficiency. The present invention has been completed by confirming that high NK cell culture efficiency can be achieved by a method of culturing a biological sample containing mononuclear cells in a cell culture vessel, a part or a whole of a surface of the cell culture vessel to be brought into contact with a biological sample being formed of a cycloolefin polymer, a part or a whole of the surface of the cell culture vessel to be brought into contact with a biological sample being coated with an anti-CD3 antibody and an anti-CD52 antibody.

Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.

The present invention is directed to compositions and methods to increase the expression of PD-L1 and/or IDO-1 in a population of cells, the modulated cells expressing increased PD-L1 and/or IDO-1, and methods related to the immunosuppressive effects obtained by cells expressing increased PD-L1 and/or IDO-1.

The present invention generally relates to novel preparations of mesenchymal stromal cells (MSCs) derived from hemangioblasts, methods for obtaining such MSCs, and method sof treating a pathology using such MSCs. The methods of the present invention produce substantial numbers of MSCs having a potency-retaining youthful phenotype, which are useful in the treatment of pathologies.

Provided is a method of preparing a hydrogel composition including a uniform content of calcium phosphate, wherein a hydrogel composition prepared by the method has a uniform content of calcium phosphate, and thus may be used to quantify phosphates contained in the hydrogel composition. Provided is an in-vitro 3D osteoporosis model including a calcium phosphate hydrogel composition, wherein osteoblasts and osteoclasts may be three-dimensionally co-cultured inside a biogel, such that the osteoporosis model may be fabricated according to an intended use or clinical stage. Further, the model contains a calcium phosphate hydrogel with a uniform content of phosphate and thus enables quantification of calcium phosphate through measurement of phosphates, and therefore, the model may be used to screen candidate compounds for an osteoporosis drug and may effectively predict therapeutic effects of the drug on osteoporosis.

The present disclosure provides engineered thymic epithelial cells and cell lines, as well as extracellular vesicles derived therefrom. The disclosure also provides exosomes that display specific surface proteins, and provides methods for using said materials for treating subjects and for identifying cells.

Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.

Compositions and methods of modulating the immune system generally involve immunomodulatory exosomes derived from platelet rich plasma subjected to multiple freeze-thaw cycles and lyophilization. In one or more embodiments, the methods involve administering the composition to a subject in an amount effective to modulate the subject's immune system. In other embodiments, the methods involve culturing a hematopoietic progenitor cell or cell differentiated from a hematopoietic progenitor cell with an immunomodulatory exosome to produce treated cells. The treated cells are then administered to the subject in an amount effective to modulate the subject's immune system.

The present disclosure relates to engineered cells and uses thereof for treating neurological disorders.