The present disclosure relates to fibers prepared using decellularized liver ECM. The disclosure further provides in vitro cultures of human hepatocytes on decellularized liver ECM fibers, and the use of the cultures in developing and screening drugs.

Engineered multilevel cell sheet-derived blood vessels and methods of preparing and using them are disclosed. Blood vessels are generated by wrapping cell sheets around a rod-like device, such as an angiocath needle, to form a tube, which is stabilized with a cyanoacrylate membrane or fibrin glue followed by endothelialization. Such engineered blood vessels can be implanted in tissue and used in vascular surgery as vascular bypass or interposition grafts as well as for vascularization and perfusion of tissue or organs prior to transplant.

The invention relates to a reconstructed scalp model, to the process for preparing it and to its use for evaluating the effect of cosmetic, pharmaceutical or dermatological topical products. The reconstructed scalp according to the invention may also be used for the preparation of the grafts intended for treating cutaneous scalp disorders.

The present invention relates to a 3-Dimensional (3D) tissue culture aggregate of cells derived from a neoplastic tissue sample, wherein 30% of total number cells are cells capable of interfering with re-aggregation. It also relates to a method of making such a 3D aggregate and a method for assessing the effectiveness of an anti-neoplasm treatment by measuring the effect of said treatment on the viability of a three dimensional (3D) neoplasm tissue culture aggregate.

The present invention relates to a method for in vitro expansion of erythroid cells. The method includes subjecting erythroid cells to 3-dimensional packed cell culture using a porous structure. The use of the composition according to the present invention enables in vitro expansion of erythroid cells in the most efficient manner.

A therapeutic serum suitable for inclusion in a cosmetic preparation may be produced by stressing a co-culture including proliferative cells. The co-culture of cells may be obtained by growing first culture to less than one-hundred percent confluence on a surface. After a monolayer of first culture is established, a second culture may be seeded onto at least one cell free area on the surface, the resulting co-culture grown to less than one-hundred percent confluence. Additional cultures may then be seeded onto cell free areas of the surface and established until a monolayer having the desired population of cells is obtained. The monolayer is then stressed to obtain a serum by conditioning a collection medium. The obtained serum may be combined with a suitable cosmetic base to provide a cosmetic preparation.

The present disclosure provides a method of producing and expanding human pancreas progenitor cells using, for example, iPSC derived cells and a human feeder cell conditioned medium. In one embodiment, cardiac mesenchyme cells are employed as feeder cells and those cells secrete growth factors, such as one or more of FGF10, KGF, or EGF, that promote pancreatic bud formation and expansion during development. In one embodiment, feeder cells are isolated from human stem cells, e.g., a human iPS-derived cardiac cells, and used to condition media and promote the growth and proliferation of iPSc derived pancreatic progenitor cells (in a feeder-free system).

The present disclosure relates to compositions and methods for culturing a population of hepatocytes in vitro, comprising co-culturing the population of hepatocytes with at least one non-parenchymal cell population and incubating the co-culture in culture medium, wherein the co-culture is periodically incubated in culture medium that does not comprise serum (serum-free culture medium).

Methods for mimicking a tumor microenvironment in vitro are provided. The methods comprise indirectly applying a shear stress upon at least one tumor cell type plated on a surface within a cell culture container. Methods for mimicking tumor metastasis and methods for testing drugs or compounds in such systems are also provided.

The present invention discloses a method for culturing human limbal stem cells comprising the steps of: pre-treating a feeder cell; seeding the feeder cells in a porous membrane; placing the porous membrane in a container to separate the container into a first cell culture compartment and a second cell culture compartment, wherein the feeder cells are located in the second cell culture compartment; injecting the culture medium into the container; and placing the human limbal stem cells in the first cell culture compartment. Compared to the prior art, the method for culturing human limbal stem cells of the present invention utilizes the porous membrane to culture the human limbal stem cells to make the expansion rate better than the traditional culture method. In addition, the culture medium used in the present invention does not contain the dimethyl sulfoxide (DMSO) with cytotoxicity to reduce the probability of cell death.