The present disclosure generally relates, in certain aspects, to cultivated meat and other cultivated animal-derived products. In some embodiments, such products may include fat replicas, which may improve taste, appearance, etc. In some cases, a fat replica can be formed by forming an emulsion of fat and a non-human blood plasma, then causing the blood plasma to crosslink and/or clot, e.g., forming a hydrogel containing the fat emulsion. In some cases, the fat replica may be used to make a cultivated meat product, e.g., by combining with muscle replicas, lysate of non-human red blood cells, etc. Other embodiments are generally directed to methods of making or using such fat replicas, the microcarriers, or the cultivated meat products, kits involving these, or the like.

The present disclosure generally relates, in certain aspects, to cultivated meat and other cultivated animal-derived products. In some embodiments, muscle and/or fat cells can be grown on microcarriers or other scaffolds, for example, in a bioreactor or other in vitro cell culture system. The microcarriers or other scaffolds can comprise materials such as fibrin. The fibrin may be formed into hydrogels or other articles, which may be edible in some cases. The microcarriers may also contain grooves or other structures in some instances. In certain embodiments, the microcarriers may be present within the final product, e.g., in a cultivated meat product. Other embodiments are generally directed to methods of making or using microcarriers or cultivated meat products, kits involving these, or the like.

The present disclosure generally relates, in certain aspects, to cultivated meat and other cultivated animal-derived products. In some embodiments, the whole blood of a non-human animal is separated into various components (e.g., concentrated red blood cells, blood plasma, etc.). Some embodiments are generally directed toward incorporating coloring or redness into a product. For example, colorants may be added to a cultivated meat product to improve its color. The colorant may comprise a lysate of non-human red blood cells, e.g., containing hemoglobin. In some embodiments, the lysate may be obtained from blood withdrawn from living animal donors. Other embodiments yet are generally directed toward the composition and method of use of the lysate, cultivated meat products, kits involving these, or the like.

The present invention is encompassed in the field of biomedicine and more specifically tissue engineering. It relates specifically to an in vitro method for preparing an artificial tissue, to the artificial tissue obtainable by said method and to the use of this artificial tissue to partially or completely increase, restore or replace the functional activity of a damaged tissue or organ.

A cell culture system including a silk fibroid scaffold, culture media, and salivary gland cells. The salivary gland cells grown in the tissue culture system have physiological and morphological features like those of in vivo salivary gland cells. The cell culture system can be used to produce a salivary tissue-specific extracellular matrix capable of inducing differentiation of salivary gland cell precursors into salivary gland cells.

The present invention provides a process or preparing an extracellular matrix composition which comprises: (a) mixing an aqueous solution of fibrinogen with a coagulating agent and a bulking agent and a foaming agent; (b) causing the mixture to foam and coagulate; (c) incubating the mixture obtained in step (b) with a cross-linking agent; and (d) washing the cross-linked composition obtained in step (c) to remove the cross-linking agent. Wherein the foaming agent consists of or comprises one or more surfactant agent(s) from the class of sugar-surfactants. The invention also relates to the formulation mixture as such, and to the products of the process.

A vascularized full thickness tissue engineered skin assembled by hydrogel, nanofibrous scaffolds and skin cell layers and a preparation method thereof relate to a technical field of polymer materials and biomedical materials. The artificial tissue engineered skin includes an epidermis layer and a dermis layer. The epidermal layer is formed by alternately stacking upper nanofibrous scaffolds located above the dermis layer and a kind of seed cells. The dermis layer is formed by lower nanofibrous scaffolds, the hydrogel layer above the lower nanofibrous scaffolds, and three kinds of seed cells distributed on surfaces of the lower nanofibrous scaffolds as well as inside and on a surface of the hydrogel layer. The artificial tissue engineered skin is prepared by a combination of electrospinning technology, polymer complexation technology and fiber/cell layer-gel layer-fiber/cell layer self-assembly technology. The bio-functional artificial tissue engineered skin can be used for regeneration and repair of various tissues.

A method of producing three-dimensional cellular tissues includes: obtaining a stromal cell-containing mixture containing stromal cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, or a stromal cell-containing mixture containing stromal cells, a cationic substance and a fragmented extracellular matrix component; gelling the stromal cell-containing mixture to obtain a first gel composition containing stromal cells; obtaining a target cell-containing mixture containing target cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, or a target cell-containing mixture containing target cells, a cationic substance and a fragmented extracellular matrix component; placing the target cell-containing mixture in contact with the first gel composition; gelling the target cell-containing mixture to obtain a second gel composition containing target cells; and incubating the first gel composition and the second gel composition to obtain three-dimensional cellular tissues.

Provided are a support for culturing cells, a method of preparing the support, and a method of culturing cells using the support. When the support and the methods are used, an adherence rate, a surface area, and a proliferation rate of cells may improve, and detachment of the cells may be facilitated, thereby increasing a cell recovery rate. In addition, a physiologically active substance may be slowly released, thereby reducing the cost for a cell culture process.

The present disclosure provides a method of deriving and maintaining a midbrain-like organoid in culture, comprising (a) culturing pluripotent stem cells to obtain neuronal lineage embryoid bodies; (b) culturing the neuronal lineage embryoid bodies from (a) to obtain midbrain regionalized tissues; (c) embedding and culturing the midbrain regionalized tissues from (b) in an extracellular matrix to obtain neuroepithelial tissues; and (c) culturing the neuroepithelial tissues from (c) to obtain a midbrain-like organoid. Also disclosed herein are culture media suitable for deriving and maintaining neuronal lineage embryoid bodies comprising (a) TGF- Inhibitor and/or SMAD2/3 inhibitors; and (b) WNT-signaling activator; culture media suitable for deriving and maintaining midbrain regionalized tissues comprising (a) TGF- Inhibitor and/or SMAD2/3 inhibitors; (b) WNT-signaling activator; (c) hedgehog signaling protein; and (d) fibroblast growth factor; culture media suitable for deriving and maintaining neuroepithelial tissues comprising (a) hedgehog signaling protein; and (b) fibroblast growth factor and culture media suitable for deriving and maintaining a midbrain-like organoid comprising (a) neurotrophin factor; (b) ascorbic acid; and (c) activator of cAMP-dependent pathway.