The present invention relates to a composition containing injectable mesenchymal stem cell-hydrogel and a method of preparing the same. Specifically, in a mesenchymal stem cell-hydrogel composition for injection prepared by a method of the present invention, since stem cells are attached to scaffolds in hydrogel beads, the stem cells are not easily lost or killed after the injection, and thus there is an advantage that an engraftment rate increases since the paracrine effect of the stem cells is continuously exhibited, and the stem cells are gradually released as hydrogel is degraded. In addition, the present invention has an advantage that healthy cells can be used without damages in cell membranes since injection formulation can be prepared without a treatment with proteolytic enzymes, and also a cryopreservation solution is easily removed from the mesenchymal stem cell-hydrogel beads even after freezing and thawing.

Disclosed is a cell structure comprising: a fragmented extracellular matrix component; and cells, wherein the cell structure comprises an intercellular vascular network, and the cells comprise at least adipocytes and vascular endothelial cells.

An object of the present invention is to provide a novel method for producing a biological tissue-like structure comprising differentiated cells induced from pluripotent stem cells, and the present invention provides a method of forming a biological tissue-like structure together with host-derived blood vessels and connective tissue by transplanting a composition in which cells derived from pluripotent stem cells are disposed to be dispersed in a biocompatible material to induce the differentiation of the cells.

There is provided a tissue scaffold and a method for making a tissue scaffold. The tissue scaffold comprises elastin and optionally fibrin and/or collagen. The elastin in the scaffold may be cross-linked. The elastin that is cross-linked preferably comprises solubilised elastin and is unfractionated.

A tissue graft for soft tissue repair or reconstruction comprising a sheet of a biopolymer-based matrix having a plurality of small perforations and a plurality of large perforations. The small perforations are sized to facilitate clotting and granulation tissue development within the perforations which, in turn, facilitates revascularization and cell repopulation in the patient. The large perforations are sized to reduce the occurrence of clotting and granulation tissue development within the perforations so that extravascular tissue fluids accumulating at the implant site can drain through the tissue graft. The large perforations enhance mammal tissue anchoring by permitting mammal tissue to compress into the perforations increasing mammal tissue contact area.

A renal tube assay device can include: a container having an inlet port and an outlet port; a matrix material in the container; and a lumen in the matrix material extending from the inlet port to the outlet port. The lumen can include a luminal surface with at least one low density region that has a lower density compared to another adjacent region of the matrix material that is located at least partially around the at least one low density region. The low density region can have a form of a bubble, bulge, capsule, or the like. The low density region can bulge into the lumen. A port can be adapted for receiving a pipette tip. The matrix material can include a hydrogel. The container can be located in a cell culture dish.

Disclosed is a brain blood vessel model composed of a three-dimensional tissue containing defibrated extracellular matrix components and cells including brain microvascular endothelial cells, pericytes, and astrocytes, wherein at least a portion of the above-described cells adheres to the above-described defibrated extracellular matrix.

The present invention concerns a method for obtaining an implantable cartilage gel for tissue repair of hyaline cartilage, comprising particles of chitosan hydrogel and cells that are capable of forming hyaline cartilage, said method comprising a step for amplification of primary cells in a three-dimensional structure comprising particles of physical hydrogel of chitosan or a chitosan derivative, then a step for re-differentiation and induction of the synthesis of extracellular matrix by said amplified cells, in the same three-dimensional structure, wherein said cells are primary articular chondrocytes and/or mesenchymal stem cells differentiated into chondrocytes. The present invention also concerns the cartilage gel obtained thereby, and its various uses for cartilage repair following a traumatic lesion or an osteoarticular disease such as osteoarthritis. The invention also concerns a three-dimensional matrix comprising particles of physical hydrogel of chitosan or of chitosan derivative, optionally supplemented with an anionic molecule such as hyaluronic acid or a derivative of hyaluronic acid or a complex of hyaluronic acid.

A device for measuring a tension of a cell structure containing muscle cells includes a first and a second gel adaptor holder. The first gel adaptor holder includes a frame member and a first gel holding portion which is disposed protrudingly from a part of an inner surface of the frame member for fixing one end of a gel. The second gel adaptor holder includes a second gel holding portion that fixes another end of the gel, and connecting members connected with the second gel holding portion. A kit including the device, a substrate and a gel forming cover. The substrate includes a pair of gel shaping convex parts fitted along the inner surface of the frame member. The gel forming cover includes a surface parallel to a gel contacting surface of the substrate, in order to form an upper surface of the gel.

The present disclosure provides compositions and methods comprising human platelet releasate (hPR), a xeno-free media supplement. The disclosure also relates to a cGMP process for the rapid, efficient and large-scale manufacturing of hPR that may be performed in less than 4 hours. The platelet releasate of the current disclosure prevents gelation of growth media alleviating the need for heparin or other anticoagulants. Mesenchymal stem cells expanded in the presence of platelet releasate have demonstrated superior expansion rates and potency compared to commercial supplements including platelet lysates. The releasate has therapeutic and medical applications. The disclosure also relates to compositions and methods related to platelet-rich fibrin.