Provided herein are primers and probes for in-vitro detection of human parvovirus 4, including primers and probes having the sequence of SEQ ID NO: 1 or a part of it or a nucleotide having minimum 90% sequence identity with SEQ ID NO: 1; SEQ ID NO: 2 or a part of it or a nucleotide having minimum 90% sequence identity with SEQ ID NO: 2; and/or SEQ ID NO: 3 or a part of it or a nucleotide having minimum 90% sequence identity with SEQ ID NO: 3. The nucleotide sequences enable high detection of all genotypes of human parvovirus 4.