The subject of this invention is improvements in the yield and titer of biological production of muconic acid by fermentation. Increased activity of one or more enzymes involved in the muconic acid pathway leads to increased production of muconic acid.

The present invention relates to a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain, to the preparation of a long-chain dibasic acid producing strain by directed evolution of POX gene and homologous recombination, and to the production of a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain by using the strain. The present invention also relates to a strain containing a mutated promoter, wherein, when a long-chain dibasic acid is produced by fermentation of this strain, the content of the acid impurity of shorter carbon chain in the fermentation product is significantly reduced.

The present invention relates to a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain, to the preparation of a long-chain dibasic acid producing strain by directed evolution of POX gene and homologous recombination, and to the production of a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain by using the strain. The present invention also relates to a strain containing a mutated promoter, wherein, when a long-chain dibasic acid is produced by fermentation of this strain, the content of the acid impurity of shorter carbon chain in the fermentation product is significantly reduced.

The present disclosure provides methods for isolating a long chain dicarboxylic acid such as a substantially pure or pure long chain dicarboxylic acid from a fermentation broth containing microbial cells.

The present disclosure provides methods for isolating a long chain dicarboxylic acid such as a substantially pure or pure long chain dicarboxylic acid from a fermentation broth containing microbial cells.

An acetyl-CoA producing microorganism obtained by imparting at least one enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism that does not have any of the following (a), (b), (c), (d) or (e): (a) a carbon dioxide fixation cycle including an enzymatic reaction from malonyl-CoA to malonate semialdehyde or 3-hydroxypropionate; (b) a carbon dioxide fixation cycle including an enzymatic reaction from acetyl-CoA and CO.sub.2 to pyruvate; (c) a carbon dioxide fixation cycle including an enzymatic reaction from crotonyl-CoA and CO.sub.2 to ethylmalonyl-CoA or glutaconyl-CoA; (d) a carbon dioxide fixation cycle including an enzymatic reaction from CO.sub.2 to formate; or (e) at least one selected from the group consisting of malate thiokinase and malyl-CoA lyase.

The present invention relates to the fields of industrial biotechnology, renewable raw materials and production of organic acids. Specifically, the invention relates to a method of producing an organic acid or a salt thereof. Still, the present invention relates to a mixture comprising legume molasses and calcium hydroxide or a mixture obtained by combining a carbon substrate composition comprising legume molasses and an aqueous suspension comprising calcium hydroxide, for producing an organic acid or a salt thereof by a microorganism, and use of said mixture for producing an organic acid or a salt thereof. Still further, the present invention relates to a method of producing one or more products selected from the group consisting of polymers, polyesters and polylactic acids.

The present invention relates to the fields of industrial biotechnology, renewable raw materials and production of organic acids. Specifically, the invention relates to a method of producing an organic acid or a salt thereof. Still, the present invention relates to a mixture comprising legume molasses and calcium hydroxide or a mixture obtained by combining a carbon substrate composition comprising legume molasses and an aqueous suspension comprising calcium hydroxide, for producing an organic acid or a salt thereof by a microorganism, and use of said mixture for producing an organic acid or a salt thereof. Still further, the present invention relates to a method of producing one or more products selected from the group consisting of polymers, polyesters and polylactic acids.

Genetically engineered oleaginous fungi (e.g., engineered Yarrowia lipolytica) are provided for use in itaconic acid production. In some aspects, the engineered fungi comprise a transgene for expression of a cis-aconitic acid decarboxylase (CAD) enzyme and, optionally, one or more further genetic modifications. Methods and culture systems for production of itaconic acid using such fungi are also provided.

Genetically engineered oleaginous fungi (e.g., engineered Yarrowia lipolytica) are provided for use in itaconic acid production. In some aspects, the engineered fungi comprise a transgene for expression of a cis-aconitic acid decarboxylase (CAD) enzyme and, optionally, one or more further genetic modifications. Methods and culture systems for production of itaconic acid using such fungi are also provided.