An enzymatic glycerolysis method to convert an oil having a first monoacylgycerol (MAG), diacylglycerol (DAG), triacylglycerol (TAG) and fatty acid composition into a structured fat is provided. The method comprising the steps of exposing the oil to glycerol in the presence of an enzyme catalyst under conditions sufficient to convert the triacylglycerols to mono- and/or di-acylglycerols; and cooling the oil to yield the structured fat having a second monoacylgycerol, diacylglycerol, triacylglycerol and fatty acid composition, wherein the fatty acid composition of the oil is essentially retained in the structured fat. The structured fat provides a healthy substitute for saturated fats in foods.

The present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, productivity of which has been enhanced. Specifically, the present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, by producing 13-hydroxy-9(Z)-octadecenoic acid from linoleic acid in the presence of a transformed microorganism that produces a protein such as the following: (A) a protein having an amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14; (B) a protein having an amino acid sequence containing one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14, and having a linoleate 13-hydratase activity; and (C) a protein having an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13 or 14, and having a linoleate 13-hydratase activity.

A process for combined renewable 1-decene and renewable carboxylic diacid production from a fatty acid ester containing feedstock, wherein the feedstock is first subjected to metathesis reaction conditions, recovery of 1-decene and then to microbial oxidation to yield diacids in a fermentation broth. Diacids of unusual carbon chains lengths are thereby obtainable.

A process for combined renewable 1-decene and renewable carboxylic diacid production from a fatty acid ester containing feedstock, wherein the feedstock is first subjected to metathesis reaction conditions, recovery of 1-decene and then to microbial oxidation to yield diacids in a fermentation broth. Diacids of unusual carbon chains lengths are thereby obtainable.

The present application relates to recombinant microorganisms useful in the biosynthesis of unsaturated C.sub.6-C.sub.24 fatty alcohols, aldehydes, and acetates which may be useful as insect pheromones, fragrances, flavors, and polymer intermediates. The recombinant microorganisms may express enzymes or enzyme variants useful for production of and/or may be modified to downregulate pathways to shunt production toward unsaturated C.sub.6-C.sub.24 fatty alcohols, aldehydes, and acetates. The C.sub.6-C.sub.24 fatty alcohols, aldehydes, and acetates described herein may be used as substrates for metathesis reactions to expand the repertoire of target compounds and pheromones. The application further relates to recombinant microorganisms co-expressing a pheromone pathway and a pathway for the production of a toxic protein, peptide, oligonucleotide, or small molecule suitable for use in an attract-and-kill pest control approach. The application further relates to microorganisms modified to express or downregulate enzymes useful for production of unsaturated short chain fatty alcohols, aldehydes, and acetates which may be useful as insect pheromones, fragrances, flavors, and polymer intermediates. Also provided are methods of producing unsaturated C.sub.6-C.sub.24 fatty alcohols, aldehydes, and acetates using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally one or more of the product alcohols, aldehydes, or acetates.

This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.

This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.

The present invention relates to a process for valorization of low-cost alkane rich feedstock. More specifically, the present invention relates to the selective conversion of alkane rich kerosene to value-added products like mono/dicarboxylic acid, fatty acids and biosurfactants using mutant yeast strain and a heterogenous nano-catalyst. The present invention also provides a mutant yeast strain for selective conversion of alkane rich refinery stream from a substrate containing hydrocarbons. The mutant yeast strain of the present invention is able to consume the sulfur content in the feed and results in the desulfurization of the alkane rich feedstock.

The present invention relates to a process for valorization of low-cost alkane rich feedstock. More specifically, the present invention relates to the selective conversion of alkane rich kerosene to value-added products like mono/dicarboxylic acid, fatty acids and biosurfactants using mutant yeast strain and a heterogenous nano-catalyst. The present invention also provides a mutant yeast strain for selective conversion of alkane rich refinery stream from a substrate containing hydrocarbons. The mutant yeast strain of the present invention is able to consume the sulfur content in the feed and results in the desulfurization of the alkane rich feedstock.

Provided is a method for producing a yeast cell wall-derived decomposition product-containing composition, the method including enzymatically treating a cell wall of yeast with exoglucanase.