The present invention relates to a process for producing enriched algal biomass having high lipid productivity. More particularly, the present invention provides a process for obtaining an enriched biomass with omega-3 fatty acids by using a microalgal strain Schizochytrium MTCC 5980 in a unique media composition and substrate residual band in a steady state continuous fermentation. The process of the present invention results in high biomass and lipid productivity.

The present disclosure relates to methods for production of (Z)-11-hexadecen-1-ol in a yeast cell using desaturases and fatty acyl-CoA reductase. Also disclosed are methods for production of (Z)-11-hexadecenal in a yeast cell. Also disclosed are methods for production of (Z)-11-hexadecen-1-yl acetate in a yeast cell. The disclosure also provides for nucleic acid constructs and yeast cells useful for performing the present methods, as well as to pheromone compositions.

The present disclosure relates to methods for production of (Z)-11-hexadecen-1-ol in a yeast cell using desaturases and fatty acyl-CoA reductase. Also disclosed are methods for production of (Z)-11-hexadecenal in a yeast cell. Also disclosed are methods for production of (Z)-11-hexadecen-1-yl acetate in a yeast cell. The disclosure also provides for nucleic acid constructs and yeast cells useful for performing the present methods, as well as to pheromone compositions.

Provided are microalgae of the genus Euglena that fall under Euglena gracilis strain EOD-1 (Accession No. FERM BP-11530) or its mutant strain and that are capable of producing at least polysaccharides. Further provided is a method for producing polysaccharides including: culturing microalgae of the genus Euglena that fall under Euglena gracilis strain EOD-1 (Accession No. FERM BP-11530) or its mutant strain and that are capable of producing at least polysaccharides as polysaccharide-producing organisms to produce the polysaccharides. Further provided is a method for producing an organic compound including: culturing microalgae of the genus Euglena that fall under Euglena gracilis strain EOD-1 (Accession No. FERM BP-11530) or its mutant strain and that are capable of producing at least polysaccharides to produce at least one organic compound selected from the group consisting of polysaccharides, lipids, vitamin C, vitamin E, pigments, and proteins.

It has been found that esterification of a hydroxy-fatty acid by a lipase can be coupled with oleate hydratase (OHase) generation of that hydroxy-FA from an unsaturated FA with a cis C9-C10 double bond, e.g. oleic acid, in a single aqueous buffered reaction medium at low temperature, e.g. 30° C. A simple one-pot enzymatic method to produce fatty acid estolides from one or more triglycerides, e.g. starting from a natural plant oil, is thereby enabled in which the same lipase catalyses both the initial hydrolysis of triglyceride and the final esterification step.

It has been found that esterification of a hydroxy-fatty acid by a lipase can be coupled with oleate hydratase (OHase) generation of that hydroxy-FA from an unsaturated FA with a cis C9-C10 double bond, e.g. oleic acid, in a single aqueous buffered reaction medium at low temperature, e.g. 30° C. A simple one-pot enzymatic method to produce fatty acid estolides from one or more triglycerides, e.g. starting from a natural plant oil, is thereby enabled in which the same lipase catalyses both the initial hydrolysis of triglyceride and the final esterification step.

The present disclosure provides a sophorolipid composition that can be used for inducing protein expression in a fermentation host. The sophorolipid composition described herein can be prepared from a natural sophorolipid mixture. Acid treatment of the natural sophorolipid mixture results in a mixture of monoacetylated, deacetylated, and/or diacetylated sophorolipids. The chemically modified sophorolipid composition, or isolated components of the chemically modified sophorolipid composition, can be used as inducers for protein production in filamentous fungi.

The disclosure provides artificially linked acyl earner proteins that enhance fatty acid biosynthesis and methods for mating the artificially linked acyl earner proteins. The fused dimer comprises acyl carrier proteins and peptide linkers.

[Problems] To provide a method of producing a lipid, containing enhancing productivity of medium chain fatty acids or the lipid containing these medium chain fatty acids as components.

[Means to solve] A method of producing a lipid, containing the steps of: culturing a transformant in which a gene encoding any one of the following proteins (A) to (C) is introduced into a host, and collecting a lipid from the cultured product: (A) a protein consisting of the amino acid sequence of the 91st to 348th positions set forth in SEQ ID NO: 1; (B) a protein consisting of an amino acid sequence having 80% or more identity with the amino acid sequence of the 91st to 348th positions set forth in SEQ ID NO: 1, and having acyl-ACP thioesterase activity; and (C) a protein containing; the amino acid sequence of the protein (A) or (B), and having acyl-ACP thioesterase activity.

[Problems] To provide a method of producing a lipid, containing enhancing productivity of medium chain fatty acids or the lipid containing these medium chain fatty acids as components.

[Means to solve] A method of producing a lipid, containing the steps of: culturing a transformant in which a gene encoding any one of the following proteins (A) to (C) is introduced into a host, and collecting a lipid from the cultured product: (A) a protein consisting of the amino acid sequence of the 611th to 772nd positions set forth in SEQ ID NO: 1; (B) a protein consisting of an amino acid sequence having 80% or more identity with the amino acid sequence of the 611th to 772nd positions set forth in SEQ ID NO: 1, and having acyl-ACP thioesterase activity; and (C) a protein containing the amino acid sequence of the protein (A) or (B), and having acyl-ACP thioesterase activity.

[Selected Drawing] None