Provided is a method for producing L-methionine in which O-phospho-L-homoserine and methanethiol are enzymatically converted into L-methionine and H3PO4. Such a conversion is achieved by an enzyme called O-phospho-L-homoserine (OHPS) dependent methionine synthase. Also described are O-phospho-L-homoserine (OHPS) dependent methionine synthases, i.e. proteins which are able to enzymatically convert O-phospho-L-homoserine and methanethiol into L-methionine and H3PO4 as well as microorganisms which have been genetically modified so as to be able to produce L-methionine from O-phospho-L-homoserine and methanethiol. Furthermore described are methods to screen for enzymes that catalyze the conversion of O-phospho-L-homoserine and methanethiol into L-methionine and H.sub.3PO.sub.4.

A dietary supplement that may include chromium 4-hydroxyisoleucinate. The chromium 4-hydroxyisoleucinate may be provided in a molar ratio between 1:5 and 5:1 ligand to metal. The dietary supplement may be used to manage blood glucose levels, to improve insulin resistance, and to improve glucose uptake.

Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.

Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.

The present invention is related to a recombinant microorganism optimised for the fermentative production of methionine and/or its derivatives, wherein in said recombinant strain, the methionine efflux is enhanced by overexpressing the homologous logous genes of ygaZ and ygaH genes from Escherichia coli. It is also related to a method for optimising the fermentative production of methionine or its derivatives comprising the steps of: a. culturing a recombinant microorganism wherein in said microorganism, the methionine efflux is enhanced by overexpressing the ygaZH homologous genes of ygaZ and ygaH genes from Escherichia coli, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and b. recovering methionine and/or its derivatives from the culture medium.

The present invention is related to a recombinant microorganism optimised for the fermentative production of methionine and/or its derivatives, wherein in said recombinant strain, the methionine efflux is enhanced by overexpressing the homologous logous genes of ygaZ and ygaH genes from Escherichia coli. It is also related to a method for optimising the fermentative production of methionine or its derivatives comprising the steps of: a. culturing a recombinant microorganism wherein in said microorganism, the methionine efflux is enhanced by overexpressing the ygaZH homologous genes of ygaZ and ygaH genes from Escherichia coli, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and b. recovering methionine and/or its derivatives from the culture medium.

The present disclosure relates to a method for preparing L-cysteine hydrochloride hydrate crystals, and L-cysteine hydrochloride hydrate crystals prepared by the method. Through the method for preparing L-cysteine hydrochloride hydrate crystals of the present disclosure, L-cysteine hydrochloride hydrate crystals can be obtained from a natural L-cysteine fermentation broth with a high recovery rate and/or purity without a chemical reaction or the use of an artificial synthetic compound.

The present invention relates to use of inducible promoters in the production of methionine by fermentation. The present invention concerns a method for the production of methionine, its precursors or derivatives in a fermentative process comprising the following steps: culturing a modified microorganism in an appropriate culture medium comprising a source of carbon, a source of sulphur and a source of nitrogen, and recovering methionine and/or its derivatives from the culture medium,
wherein in said modified microorganism, the expression of at least one gene involved in methionine production is under the control, direct or indirect, of a heterologous inducible promoter. The invention also concerned the modified microorganism used in the method.

The present invention relates to use of inducible promoters in the production of methionine by fermentation. The present invention concerns a method for the production of methionine, its precursors or derivatives in a fermentative process comprising the following steps: culturing a modified microorganism in an appropriate culture medium comprising a source of carbon, a source of sulphur and a source of nitrogen, and recovering methionine and/or its derivatives from the culture medium,
wherein in said modified microorganism, the expression of at least one gene involved in methionine production is under the control, direct or indirect, of a heterologous inducible promoter. The invention also concerned the modified microorganism used in the method.

The present invention describes an alternative approach to increase methionine (Met) production in eukaryotes, namely by the insertion of components of a sulfur-metabolic pathway in organisms where the pathway does not exist or has not clearly been identified. The invention describes methods for the use of polynucleotides that encode functional cysteine dioxygenase (CDO) alone or CDO and sulfinoalanine decarboxylase (SAD) polypeptides in plants to increase Met production. The preferred embodiment of the invention is in plants but other organisms may be used. Changes in Met availability will improve nutritional value of the crop.