A method for detecting and characterizing a microorganism in a clinical sample includes introducing a clinical sample to a first culture vessel containing the culture medium; removing a test aliquot; separating DNA from the test aliquot; and performing nucleic acid tests on the DNA to identify the microorganism and to detect the presence or absence of one or more genetic antimicrobial resistance markers in the microorganism. If a microorganism is identified, an antimicrobial susceptibility test is performed wherein microbial growth in the antimicrobial susceptibility test is monitored by accessing growth or markers for growth and wherein the type and concentration of antimicrobial agents used in the antimicrobial susceptibility test is determined by the identity of the microorganism and the antimicrobial resistance markers detected. A device for performing the method is also provided.

Probes for the detection of β-lactamase-type enzymatic activity. In particular, novel fluorogenic substrates for detecting the presence of a catalytically active β-lactamase and a detection method using such substrates.

Methods for processing and analyzing samples are presented, said methods useful for, among other uses, analysis of lipids and other analytes of a sample, said methods further useful for identification of microorganisms at the level of species, identification of microorganisms at a level other than species, detecting infections and other diseases, detecting and measuring growth of an organism, detecting and measuring an environmental response of an organism, determining and/or measuring antimicrobial resistance of a microorganism, and for other purposes.

Methods for processing and analyzing samples are presented, said methods useful for, among other uses, analysis of lipids and other analytes of a sample, said methods further useful for identification of microorganisms at the level of species, identification of microorganisms at a level other than species, detecting infections and other diseases, detecting and measuring growth of an organism, detecting and measuring an environmental response of an organism, determining and/or measuring antimicrobial resistance of a microorganism, and for other purposes.

The invention enables efficient, rapid, and sensitive enumeration of living cells by detecting microscopic colonies derived from in situ cell division using large area imaging. Microbial enumeration tests based on the invention address an important problem in clinical and industrial microbiology—the long time needed for detection in traditional tests—while retaining key advantages of the traditional methods based on microbial culture. Embodiments of the invention include non-destructive aseptic methods for detecting cellular microcolonies without labeling reagents. These methods allow for the generation of pure cultures which can be used for microbial identification and determination of antimicrobial resistance.

The invention enables efficient, rapid, and sensitive enumeration of living cells by detecting microscopic colonies derived from in situ cell division using large area imaging. Microbial enumeration tests based on the invention address an important problem in clinical and industrial microbiology—the long time needed for detection in traditional tests—while retaining key advantages of the traditional methods based on microbial culture. Embodiments of the invention include non-destructive aseptic methods for detecting cellular microcolonies without labeling reagents. These methods allow for the generation of pure cultures which can be used for microbial identification and determination of antimicrobial resistance.

Described herein are methods of treating COVID19 from a coronavirus infection, methods of treating a subject having a coronavirus infection, methods of improving a survival rate of a subject having a coronavirus infection, methods of determining prognosis of a subject having a coronavirus infection, methods of preventing or treating organ-dysfunction or multi-organ dysfunction or failure associated with a coronavirus infection, methods of combinational therapy for a subject having a coronavirus infection, methods of reducing microthrombi formation or low flow organ-ischemia associated with a coronavirus infection by administering a DEspR inhibitor.

A polymer having a first monomer operatively connected to Rose Bengal, a second monomer, and a surfactant, wherein the surfactant is selected from the group consisting of ionic surfactants, anionic surfactants, cationic surfactants, nonionic surfactants, zwitterionic surfactants, and mixtures thereof. The Rose Bengal in polymer in an amount effective for rendering the polymer antimicrobial or antiviral upon exposure of said polymer to light; and the polymer produces singlet oxygen from air in the presence of light. A substrate have these features is also included.

Methods and compositions for detecting an antibiotic-inactivating factor produced by a microorganism are described herein.

Methods and compositions for detecting an antibiotic-inactivating factor produced by a microorganism are described herein.