C12Q1/24

METHOD FOR OBTAINING ENDOMETRIAL MESENCHYMAL STEM CELLS FROM HUMAN MENSTRUAL BLOOD
20230116841 · 2023-04-13 ·

Provided are a method for obtaining endometrial mesenchymal stem cells from human menstrual blood and the endometrial mesenchymal stem cells prepared thereby. The method include: 1) subjecting an aliquot of menstrual blood to cascade filtration through graded cell strainers of decreasing mesh sizes to separate tissue debris, wherein said cell strainers include a filter-through cell strainer for collecting filtrate and a retention cell strainer for collecting retentate, wherein the mesh size of the retention cell strainer is smaller than that of the filter-through cell strainer; 2) collecting the retentate on the retention cell strainer as the tissue debris; 3) culturing the tissue debris in a mesenchymal stem cells medium; and 4) collecting adherent cells as the endometrial mesenchymal stem cells.

METHOD FOR OBTAINING ENDOMETRIAL MESENCHYMAL STEM CELLS FROM HUMAN MENSTRUAL BLOOD
20230116841 · 2023-04-13 ·

Provided are a method for obtaining endometrial mesenchymal stem cells from human menstrual blood and the endometrial mesenchymal stem cells prepared thereby. The method include: 1) subjecting an aliquot of menstrual blood to cascade filtration through graded cell strainers of decreasing mesh sizes to separate tissue debris, wherein said cell strainers include a filter-through cell strainer for collecting filtrate and a retention cell strainer for collecting retentate, wherein the mesh size of the retention cell strainer is smaller than that of the filter-through cell strainer; 2) collecting the retentate on the retention cell strainer as the tissue debris; 3) culturing the tissue debris in a mesenchymal stem cells medium; and 4) collecting adherent cells as the endometrial mesenchymal stem cells.

Bioassisted treatment of microbiologically influenced corrosion in petroleum transporting pipelines

The present invention relates to a method for reducing or preventing the formation or activity of a corrosion-associated biofilm on a metal surface, wherein the method comprises contacting the metal surface with a liquid composition comprising biocidal preparation. The present invention also relates to a microbicidal composition comprising at least one alcohol, one liquid hydrocarbon, a bacteriophage immobilized on a magnetic nanocomposite, at least one phage releasing reagent and one stabilizer. The microbicide composition and method of the present invention reduces biofilms on surfaces, and consequently, reduces, mitigates, or eliminates MIC in internal surface of the oil transporting pipelines.

Bioassisted treatment of microbiologically influenced corrosion in petroleum transporting pipelines

The present invention relates to a method for reducing or preventing the formation or activity of a corrosion-associated biofilm on a metal surface, wherein the method comprises contacting the metal surface with a liquid composition comprising biocidal preparation. The present invention also relates to a microbicidal composition comprising at least one alcohol, one liquid hydrocarbon, a bacteriophage immobilized on a magnetic nanocomposite, at least one phage releasing reagent and one stabilizer. The microbicide composition and method of the present invention reduces biofilms on surfaces, and consequently, reduces, mitigates, or eliminates MIC in internal surface of the oil transporting pipelines.

ARTICLE USEABLE FOR SAMPLING SURFACES FOR MICROORGANISMS AND METHODS OF USE

Article for sampling comprising inner layer, one or more outer layers, and optional scouring layer on a portion of the one or more outer layers.

ARTICLE USEABLE FOR SAMPLING SURFACES FOR MICROORGANISMS AND METHODS OF USE

Article for sampling comprising inner layer, one or more outer layers, and optional scouring layer on a portion of the one or more outer layers.

METHOD FOR ANTIBIOTIC SUSCEPTIBILITY TESTING OF BLOOD SAMPLES

The present disclosure relates to a method for determining the susceptibility of a microorganism to an antimicrobial agent. In particular, to a method for testing the antimicrobial susceptibility of blood samples in patients with a suspected bloodstream infection. In one aspect, for example, the method comprises the steps of: (a) incubating a first blood culture derived from the blood sample; (b) terminating the incubation of the first blood culture after a period of up to about 20 hours from commencement of Step (a) to produce an incubated culture; and (c) performing a molecular phenotypic antimicrobial susceptibility test (AST) assay on the incubated culture to determine the antimicrobial susceptibility of a microorganism.

FILTRATION UNIT, METHOD FOR PRODUCING SAME, METHOD FOR DETECTING MICROORGANISMS AND USE OF THE FILTRATION UNIT

The present invention relates to a filtration unit with a filtration membrane, nutritive cardboard disc and, if necessary, a support structure, wherein the nutritive cardboard disc and/or the support structure comprises a solid, water-soluble nutrient medium and a water-soluble and/or water-swellable polymer, a method for producing the filtration unit, a method for detecting microorganisms in a fluid, wherein the filtration unit is used, and the use of the filtration unit for detecting microorganisms in a fluid.

SPECTROMETRIC ANALYSIS OF MICROBES

A method of analysis using mass spectrometry and/or ion mobility spectrometry is disclosed. The method comprises: using a first device to generate smoke, aerosol or vapour from a target comprising or consisting of a microbial population; mass analysing and/or ion mobility analysing said smoke, aerosol or vapour, or ions derived therefrom, in order to obtain spectrometric data; and analysing said spectrometric data in order to analyse said microbial population.

SPECTROMETRIC ANALYSIS OF MICROBES

A method of analysis using mass spectrometry and/or ion mobility spectrometry is disclosed. The method comprises: using a first device to generate smoke, aerosol or vapour from a target comprising or consisting of a microbial population; mass analysing and/or ion mobility analysing said smoke, aerosol or vapour, or ions derived therefrom, in order to obtain spectrometric data; and analysing said spectrometric data in order to analyse said microbial population.