The present invention relates to compositions comprising: a polypeptide, wherein at least a portion of the polypeptide has a coiled coil structure; and a chelate comprising a chelating agent and a metal ion; and wherein the chelate is bound to at least one amino acid of the polypeptide. In a preferred embodiment the polypeptide is a silk fibroin, wherein at least a portion of the silk fibroin has a coiled coil structure.

The present invention relates to compositions comprising: a polypeptide, wherein at least a portion of the polypeptide has a coiled coil structure; and a chelate comprising a chelating agent and a metal ion; and wherein the chelate is bound to at least one amino acid of the polypeptide. In a preferred embodiment the polypeptide is a silk fibroin, wherein at least a portion of the silk fibroin has a coiled coil structure.

The subject invention provides methods, assays, and products for visual detection of small-molecule targets in a sample in both clinical and field settings within minutes. The subject invention is based on an aptamer sensor that reports the presence of small-molecule target via a sensitive colorimetric signal for naked-eye detection. The aptamer sensor is a CBSAzyme-based sensor having both target-mediated cooperative behavior of the CBSA and peroxidase-mimicking catalytic activity of DNAzyme. The subject invention also provides methods of using the CBSAzyme-based sensor.

Disclosed embodiments concern a composition comprising DAB chromogen, and/or derivative thereof, a stabilizer, and polymer capable of preventing or reducing DAB precipitation relative to a composition that does not comprise the polymer. Also disclosed herein is a method for using the disclosed composition and embodiments of a kit.

Disclosed embodiments concern a composition comprising DAB chromogen, and/or derivative thereof, a stabilizer, and polymer capable of preventing or reducing DAB precipitation relative to a composition that does not comprise the polymer. Also disclosed herein is a method for using the disclosed composition and embodiments of a kit.

A method for rapid, high throughput screening of the activities of enzymes, especially ligninases and its enzyme cocktails, using nanostructure initiator mass spectrometry (NIMS) surfaces, substrates and methodology.

A method for rapid, high throughput screening of the activities of enzymes, especially ligninases and its enzyme cocktails, using nanostructure initiator mass spectrometry (NIMS) surfaces, substrates and methodology.

A reagent for enhancing a chemiluminescent reaction includes luminol or luminol derivatives, an oxidant, an electron mediator, and an enhancer. The enhancer is a nitrogen-containing fused heterocyclic compound having at least two nitrogen atoms. The present disclosure further provides a kit for enhancing a chemiluminescent reaction comprising the foregoing reagent.

A reagent for enhancing a chemiluminescent reaction includes luminol or luminol derivatives, an oxidant, an electron mediator, and an enhancer. The enhancer is a nitrogen-containing fused heterocyclic compound having at least two nitrogen atoms. The present disclosure further provides a kit for enhancing a chemiluminescent reaction comprising the foregoing reagent.

The present invention pertains to: an oxidation color developable compound that is represented by general formula (1), that has excellent solubility in water, and that is less affected by a substance coexisting in a sample; and an oxidation color development reagent using the oxidation color developable compound. In general formula (I), R.sup.1, R.sup.2, R.sup.3, and R.sup.4 each represent a straight-chain or branched alkyl group having 1-6 carbon atoms, X represents a hydrophilic functional group, and L represents (CH.sub.2).sub.j (j represents an integer of 2-10), (CH.sub.2CH.sub.2O).sub.k (k represents an integer of 1-10), or (CH.sub.2).sub.mZ(CH.sub.2).sub.n (m and n each independently represent an integer of 1-10, and Z represents N.sup.+(CH.sub.3).sub.2, CONH, NHCO, COO, OCO, NHCOO, OCONH, NHCONH, and (CH.sub.2NHCO).sub.q).