A method for increasing the light emission produced by the chemiluminescent reaction of 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, a peroxidase enzyme or a conjugate thereof, an enhancer, a co-enhancer and a peroxide oxidizer, wherein the enhancer is an anionic N-alkylphenothiazine and the co-enhancer is selected from a 4-dialkylaminopyridine or an N-azole, and wherein the method comprises the following steps: i. realizing a chemiluminescent substrate by means of mixing together 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, the enhancer, the co-enhancer and the peroxide oxidizer, and ii. adding the peroxidase enzyme or a conjugate thereof to the chemiluminescent substrate.

Provided is a catalase inhibitor comprising a compound represented by formula (I): ##STR00001##
wherein R.sub.1 to R.sub.4 independently represent a hydrogen atom, a halogen atom, an amino group, a hydroxyl group, a carbonyl group, or a hydrocarbon group having 1 to 4 carbon atoms, wherein the hydrocarbon group may have at least one substituent selected from the group consisting of a halogen atom, an amino group, a hydroxyl group and a carbonyl group; and X.sup. represents an anionic chemical species.

Provided is a catalase inhibitor comprising a compound represented by formula (I): ##STR00001##
wherein R.sub.1 to R.sub.4 independently represent a hydrogen atom, a halogen atom, an amino group, a hydroxyl group, a carbonyl group, or a hydrocarbon group having 1 to 4 carbon atoms, wherein the hydrocarbon group may have at least one substituent selected from the group consisting of a halogen atom, an amino group, a hydroxyl group and a carbonyl group; and X.sup. represents an anionic chemical species.

A method is provided for measuring glycated hemoglobin in a hemoglobin-containing sample which comprises: adding an enzyme that catalyzes a reaction of oxidizing glycated amino acid or glycated peptide to generate hydrogen peroxide without oxidizing the glycated hemoglobin, to the hemoglobin-containing sample to generate hydrogen peroxide; eliminating the generated hydrogen peroxide; adding an enzyme that catalyzes a reaction of oxidizing glycated hemoglobin to generate hydrogen peroxide thereto to generate hydrogen peroxide; and measuring the generated hydrogen peroxide.

A method is provided for measuring glycated hemoglobin in a hemoglobin-containing sample which comprises: adding an enzyme that catalyzes a reaction of oxidizing glycated amino acid or glycated peptide to generate hydrogen peroxide without oxidizing the glycated hemoglobin, to the hemoglobin-containing sample to generate hydrogen peroxide; eliminating the generated hydrogen peroxide; adding an enzyme that catalyzes a reaction of oxidizing glycated hemoglobin to generate hydrogen peroxide thereto to generate hydrogen peroxide; and measuring the generated hydrogen peroxide.

To provide a pathological index for Alzheimer's disease conveniently and with high precision, an Alzheimer's disease diagnosis device that includes a measurement means configured to measure one index or more selected from the group consisting of superoxide production activity, myeloperoxidase activity, oxidized LDL level, phagocytosis, triglycerides, fasting blood glucose, total cholesterol, hemoglobin A1c, and insulin in peripheral blood and a displaying means configured to display an index measured by the measurement means as a pathological index for Alzheimer's disease.

To provide a pathological index for Alzheimer's disease conveniently and with high precision, an Alzheimer's disease diagnosis device that includes a measurement means configured to measure one index or more selected from the group consisting of superoxide production activity, myeloperoxidase activity, oxidized LDL level, phagocytosis, triglycerides, fasting blood glucose, total cholesterol, hemoglobin A1c, and insulin in peripheral blood and a displaying means configured to display an index measured by the measurement means as a pathological index for Alzheimer's disease.

A process for manufacturing particles of water-absorbing material is provided. The process includes providing a powder bed composed of an absorptive powder comprising a water-absorbing polysaccharide onto a surface; releasing an aqueous solution from a solution dispenser so as to contact the powder bed, thereby forming a solution-impregnated humid material; letting the solution-impregnated humid material agglomerate in substantially shear-less conditions to form an agglomerated humid material, the solution-impregnated humid material being supported by the surface; and drying the agglomerated humid material, thereby forming the particles.

A process for manufacturing particles of water-absorbing material is provided. The process includes providing a powder bed composed of an absorptive powder comprising a water-absorbing polysaccharide onto a surface; releasing an aqueous solution from a solution dispenser so as to contact the powder bed, thereby forming a solution-impregnated humid material; letting the solution-impregnated humid material agglomerate in substantially shear-less conditions to form an agglomerated humid material, the solution-impregnated humid material being supported by the surface; and drying the agglomerated humid material, thereby forming the particles.

The present invention relates to novel NADPH oxidase proteins, or Nox, the use thereof, the method of preparation thereof and the method for identification thereof.