Provided herein are methods for generating circular nucleic acid molecules and circular nucleic acid libraries. The methods can be used to generate clonal populations of target nucleic acid molecules for downstream applications such as sequencing. Nucleic acid sequence methods, systems and kits are also provided for sequencing circular nucleic acid molecules.

Provided herein are methods for generating circular nucleic acid molecules and circular nucleic acid libraries. The methods can be used to generate clonal populations of target nucleic acid molecules for downstream applications such as sequencing. Nucleic acid sequence methods, systems and kits are also provided for sequencing circular nucleic acid molecules.

The present application relates to an aptamer nucleic acid molecule, a complex containing the aptamer nucleic acid molecules, a method of detecting intracellular or extracellular RNA, DNA or other target molecules, and a kit containing the aptamer. The aptamer of the present application is capable of specifically binding a kind of fluorophore micromolecules, and can significantly enhance fluorescence intensity under excitation light of appropriate wavelength.

The present application relates to an aptamer nucleic acid molecule, a complex containing the aptamer nucleic acid molecules, a method of detecting intracellular or extracellular RNA, DNA or other target molecules, and a kit containing the aptamer. The aptamer of the present application is capable of specifically binding a kind of fluorophore micromolecules, and can significantly enhance fluorescence intensity under excitation light of appropriate wavelength.

The invention provides methods and materials that can be used to determine three dimensional structures of RNA hairpin loops and their complexes with inhibitors easily and quickly. The scaffold RNA, YdaO-type c-di-AMP riboswitch from Thermoanaerobacterpseudethanolicus, readily forms crystals with a large cavity over 60 in diameter. A hairpin of interest can be engineered into the P2 stem of this RNA so that the hairpin is accommodated in the cavity. The fusion RNA is then crystallized, and structures can be determined using X-ray or electron crystallography. Embodiments of the invention can be used to identify compounds that bind hairpin loops in order to, for example, effect therapeutic and other biological activities.

The invention provides methods and materials that can be used to determine three dimensional structures of RNA hairpin loops and their complexes with inhibitors easily and quickly. The scaffold RNA, YdaO-type c-di-AMP riboswitch from Thermoanaerobacterpseudethanolicus, readily forms crystals with a large cavity over 60 in diameter. A hairpin of interest can be engineered into the P2 stem of this RNA so that the hairpin is accommodated in the cavity. The fusion RNA is then crystallized, and structures can be determined using X-ray or electron crystallography. Embodiments of the invention can be used to identify compounds that bind hairpin loops in order to, for example, effect therapeutic and other biological activities.

The invention relates to methods of multiplex detection of a plurality of target nucleic acids by contacting a sample with an acid reagent to remove bound nucleic acid detection systems, thereby allowing the same detection systems to be used again to detect different target nucleic acids and to provide for higher levels of multiplexing. The invention also relates to kits containing an acid reagent and optionally probes for detection of target nucleic acids.

The invention relates to methods of multiplex detection of a plurality of target nucleic acids by contacting a sample with an acid reagent to remove bound nucleic acid detection systems, thereby allowing the same detection systems to be used again to detect different target nucleic acids and to provide for higher levels of multiplexing. The invention also relates to kits containing an acid reagent and optionally probes for detection of target nucleic acids.

Disclosed herein are primers and probes related to the detection of beta actin [Homo sapiens (human)] via nucleic acid amplification testing (NAAT), for example to amplify and determine the presence of β-actin present in test samples. Specifically, the present disclosure describes primers and probes that bind to the beta actin gene for detection via loop mediated isothermal amplification (LAMP) and molecular beacon hybridization.

Disclosed herein are primers and probes related to the detection of beta actin [Homo sapiens (human)] via nucleic acid amplification testing (NAAT), for example to amplify and determine the presence of β-actin present in test samples. Specifically, the present disclosure describes primers and probes that bind to the beta actin gene for detection via loop mediated isothermal amplification (LAMP) and molecular beacon hybridization.