The present invention relates to compositions comprising and methods of producing genetically engineered bacteriophages, bacteriophage-like particles and non-replicating transduction particles (NRTPs) that contain non-native tail fibers that display altered host specificity and/or reactivity. The present invention also relates to methods of using these bacteriophages and NRTPs for the development of novel diagnostics, therapeutics and/or research reagents for bacteria-related diseases.

According to one embodiment, a vector set includes a first vector and a second vector. The first vector includes a transposase target sequence, a first promoter sequence ligated to downstream of the transposase target sequence, and a first reporter gene ligated to downstream of the first promoter sequence. The second vector includes a 5′-side transposase recognition sequence, a 3′-side transposase recognition sequence, and a first enhancer sequence arranged therebetween.

The present invention provides methods and compositions for detecting genomic sequences of interest in living cells. In particular, the present disclosure provides a split-enzyme system that works with guide RNAs and RNA-guided nucleases to produce detectable luminescent signals exclusively in the presence of targeted genomic sequences.

The present invention provides methods and compositions for detecting genomic sequences of interest in living cells. In particular, the present disclosure provides a split-enzyme system that works with guide RNAs and RNA-guided nucleases to produce detectable luminescent signals exclusively in the presence of targeted genomic sequences.

The present invention provides methods and compositions for detecting genomic sequences of interest in living cells. In particular, the present disclosure provides a split-enzyme system that works with guide RNAs and RNA-guided nucleases to produce detectable luminescent signals exclusively in the presence of targeted genomic sequences.

The present disclosure relates to high-throughput detection of polyketides using genetically encoded biosensors.

The present disclosure relates to high-throughput detection of polyketides using genetically encoded biosensors.

Disclosed are isothiocyanate (ITC)-detecting biosensors that utilize recombinant host cells containing an ITC responsive genetic element such as a saxA promoter, operably linked with a reporter element, such as a luxCDABE operon or ilux operon. Such biosensors can detect the presence of diverse ITCs in samples such as plant extracts, biofumigated soils and seed meal amended soils.

A multiplexed and encapsulated 3D cell co-culture drug testing or screening method which discloses an in vitro drug testing kit suitable for testing the effect of one or more drugs of interest on multiple biological processes in one or more target cell types.

A multiplexed and encapsulated 3D cell co-culture drug testing or screening method which discloses an in vitro drug testing kit suitable for testing the effect of one or more drugs of interest on multiple biological processes in one or more target cell types.