A method for quantifying mRNA capping efficiency includes combining a sample, an enzyme mixture, and an isotopic standard solution in a buffer solution to create an incubation mixture, the enzyme mixture including a non-specific, single-stranded nuclease and an acid phosphatase and the isotopic standard including isotopically labeled m7G and isotopically labeled 2-O-methylated nucleoside; incubating the mixture; and analyzing the mixture using liquid chromatography-mass spectrometry to determine at least one of a capping efficiency and a 2-O-methyltransferase efficiency.

A method for quantifying mRNA capping efficiency includes combining a sample, an enzyme mixture, and an isotopic standard solution in a buffer solution to create an incubation mixture, the enzyme mixture including a non-specific, single-stranded nuclease and an acid phosphatase and the isotopic standard including isotopically labeled m7G and isotopically labeled 2-O-methylated nucleoside; incubating the mixture; and analyzing the mixture using liquid chromatography-mass spectrometry to determine at least one of a capping efficiency and a 2-O-methyltransferase efficiency.

The RNA molecule of the present invention, which is based on a helix 73-derived RNA substrate and Erm interaction sites, may be used for screening candidate substances that inhibit methylation by Erm, developing variants thereof, and identifying the action mechanism, and may be used for suppressing the expression of antibiotic resistance.

The RNA molecule of the present invention, which is based on a helix 73-derived RNA substrate and Erm interaction sites, may be used for screening candidate substances that inhibit methylation by Erm, developing variants thereof, and identifying the action mechanism, and may be used for suppressing the expression of antibiotic resistance.

The present invention relates to methods and kits for the detection of 5-hydroxymethylcytosine (5hmC) and/or 5-methylcytosine (5meC). In some embodiments, the present invention relates to methods and kits for detection of 5hmC and/or 5meC in nucleic acid (e.g., DNA, RNA). In some embodiments, the present invention relates to detection of 5hmC in genomic DNA, e.g., mammalian genomic DNA

The present invention relates to methods and kits for the detection of 5-hydroxymethylcytosine (5hmC) and/or 5-methylcytosine (5meC). In some embodiments, the present invention relates to methods and kits for detection of 5hmC and/or 5meC in nucleic acid (e.g., DNA, RNA). In some embodiments, the present invention relates to detection of 5hmC in genomic DNA, e.g., mammalian genomic DNA

Provided is a method for modifying a ssRNA at the 3 end, said method comprising contacting the strand with a ssRNA 2-O-methyltransferase in the presence of a co-factor, under conditions which allow for the transfer by the ssRNA 2-O-methyltransferase of a part of the co-factor onto 3 end of the ssRNA to form a modified ssRNA, wherein the ssRNA bears 2-OH group at 3 terminal nucleotide and wherein the part of the co-factor transferred comprises a reporter group or a functional group.

Provided is a method for modifying a ssRNA at the 3 end, said method comprising contacting the strand with a ssRNA 2-O-methyltransferase in the presence of a co-factor, under conditions which allow for the transfer by the ssRNA 2-O-methyltransferase of a part of the co-factor onto 3 end of the ssRNA to form a modified ssRNA, wherein the ssRNA bears 2-OH group at 3 terminal nucleotide and wherein the part of the co-factor transferred comprises a reporter group or a functional group.

The present invention relates to methods and kits for the detection of 5-hydroxymethylcytosine (5hmC) and/or 5-methylcytosine (5meC). In some embodiments, the present invention relates to methods and kits for detection of 5hmC and/or 5meC in nucleic acid (e.g., DNA, RNA). In some embodiments, the present invention relates to detection of 5hmC in genomic DNA, e.g., mammalian genomic DNA.

The present invention relates to methods and kits for the detection of 5-hydroxymethylcytosine (5hmC) and/or 5-methylcytosine (5meC). In some embodiments, the present invention relates to methods and kits for detection of 5hmC and/or 5meC in nucleic acid (e.g., DNA, RNA). In some embodiments, the present invention relates to detection of 5hmC in genomic DNA, e.g., mammalian genomic DNA.