The invention provides methods and compositions for generating mutations in new nucleic acid molecules through incorporation of a transformable nucleoside into the nucleic acid and subsequent transformation of the nucleoside through oxidative-nucleophilic-aromatic-substitution chemistry, referred to as TimeLapse chemistry. The invention further provides methods for detecting the mutations, referred to as TimeLapse-seq.

The invention provides methods and compositions for generating mutations in new nucleic acid molecules through incorporation of a transformable nucleoside into the nucleic acid and subsequent transformation of the nucleoside through oxidative-nucleophilic-aromatic-substitution chemistry, referred to as TimeLapse chemistry. The invention further provides methods for detecting the mutations, referred to as TimeLapse-seq.

There is provided a microchip including a reaction region and a detection region connected to the reaction region by a flow passage, the detection region including copper.

There is provided a microchip including a reaction region and a detection region connected to the reaction region by a flow passage, the detection region including copper.

An apparatus to detect more than one analyte in a solution comprising at least one electrode in contact with the solution, at least two dyes including a first dye and a second dye, and an electrochemically active agent, where the solution has a pH, the electrode is configured to modulate the pH of the solution by oxidizing or reducing the electrochemically active agent, the first dye and the second dye fluoresce at different pH levels, fluorescence of the first dye is used to indicate the presence of a first analyte, and fluorescence of the second dye is used to indicate the presence of a second analyte. Methods of detecting multiple analytes in a solution are also provided.

An apparatus to detect more than one analyte in a solution comprising at least one electrode in contact with the solution, at least two dyes including a first dye and a second dye, and an electrochemically active agent, where the solution has a pH, the electrode is configured to modulate the pH of the solution by oxidizing or reducing the electrochemically active agent, the first dye and the second dye fluoresce at different pH levels, fluorescence of the first dye is used to indicate the presence of a first analyte, and fluorescence of the second dye is used to indicate the presence of a second analyte. Methods of detecting multiple analytes in a solution are also provided.

Provided herein are methods for the rapid preparation of amplifiable nucleic acids from biological samples, which can be applied to various applications, such as, for example, point-of-care diagnostics, service laboratory diagnostics, and molecular biology applications. These methods can be performed in 15 minutes or less, and preferably in 5 minutes or less. For most applications, no further purification of nucleic acids is needed.

Provided herein are methods for the rapid preparation of amplifiable nucleic acids from biological samples, which can be applied to various applications, such as, for example, point-of-care diagnostics, service laboratory diagnostics, and molecular biology applications. These methods can be performed in 15 minutes or less, and preferably in 5 minutes or less. For most applications, no further purification of nucleic acids is needed.

The present invention relates to a composition for detecting nucleic acid comprising duplex molecular beacon and graphene oxide and a colorimetric signal enhancement method of detecting nucleic acid using the same. According to the composition, kit and method for detecting nucleic acid of the present invention, a complex can be formed by adsorbing a single strand having a DNAzyme sequence dissociated from the conjugate of a duplex molecular beacon and a target nucleic acid to the graphene oxide surface, and separated, and a colorimetric signal amplified therefrom can be induced, so that a very low concentration of target nucleic acid can be detected with high efficiency and the target nucleic acid can be detected quickly and easily in seconds. Therefore, a new colorimetric target nucleic acid detection system capable of point of care testing (POCT) can be provided.

The present invention relates to a composition for detecting nucleic acid comprising duplex molecular beacon and graphene oxide and a colorimetric signal enhancement method of detecting nucleic acid using the same. According to the composition, kit and method for detecting nucleic acid of the present invention, a complex can be formed by adsorbing a single strand having a DNAzyme sequence dissociated from the conjugate of a duplex molecular beacon and a target nucleic acid to the graphene oxide surface, and separated, and a colorimetric signal amplified therefrom can be induced, so that a very low concentration of target nucleic acid can be detected with high efficiency and the target nucleic acid can be detected quickly and easily in seconds. Therefore, a new colorimetric target nucleic acid detection system capable of point of care testing (POCT) can be provided.