The present invention relates to a probe having octamine or octamine derivative bound thereto in addition to a reporter and a quencher to thus allow the quencher to more effectively suppress light emitted by the reporter, and to uses of the probe. When the probe according to the present invention is utilized, octamine or octamine derivative bound to the probe effectively suppresses the light emitted by the quencher for the reporter, and results in effects such as i) a reduction in base fluorescence, ii) an increase in delta fluorescence, and iii) a decrease in the value of cycle at threshold, thus allowing the probe to be effectively used in a variety of real-time polymerase chain reactions requiring accuracy and sensitivity.

The present invention relates to a probe having octamine or octamine derivative bound thereto in addition to a reporter and a quencher to thus allow the quencher to more effectively suppress light emitted by the reporter, and to uses of the probe. When the probe according to the present invention is utilized, octamine or octamine derivative bound to the probe effectively suppresses the light emitted by the quencher for the reporter, and results in effects such as i) a reduction in base fluorescence, ii) an increase in delta fluorescence, and iii) a decrease in the value of cycle at threshold, thus allowing the probe to be effectively used in a variety of real-time polymerase chain reactions requiring accuracy and sensitivity.

Methods for multiplexed detection of a nucleic acid sequence in a sample including the use of a plurality of oligonucleotide target-specific probes (TSPs) configured to bind to a distinct target nucleic acid sequence, where each of the TSPs includes one or more copies of a first fluorescent probe (FP) binding region and one or more copies of a second FP binding region, and where a predetermined ratio of the one or more copies of the first FP binding region to the one or more copies of the second FP binding region is indicative of the distinct target nucleic acid sequence the TSP is configured to bind to.

Methods for multiplexed detection of a nucleic acid sequence in a sample including the use of a plurality of oligonucleotide target-specific probes (TSPs) configured to bind to a distinct target nucleic acid sequence, where each of the TSPs includes one or more copies of a first fluorescent probe (FP) binding region and one or more copies of a second FP binding region, and where a predetermined ratio of the one or more copies of the first FP binding region to the one or more copies of the second FP binding region is indicative of the distinct target nucleic acid sequence the TSP is configured to bind to.

Disclosed herein are methods and compositions for detection of one or more specific sequences of polynucleotides in a solution using a nanopore. In some embodiments, methods and compositions for identifying a polynucleotide in a sample or for target sequence detection of a polynucleotide are disclosed herein.

Disclosed herein are methods and compositions for detection of one or more specific sequences of polynucleotides in a solution using a nanopore. In some embodiments, methods and compositions for identifying a polynucleotide in a sample or for target sequence detection of a polynucleotide are disclosed herein.

The present disclosure is directed to methods and kits for detection and/or quantification of nucleic acids, such as double stranded DNA that is used in next-generation sequencing (NGS) applications. Nucleic acid-based probes, such as peptide nucleic acid (PNA) oligomers, molecular beacons, DNA flares and locked nucleic acid (LNA) oligomers, are also provided for use in the methods and kits of the present disclosure.

The present disclosure is directed to methods and kits for detection and/or quantification of nucleic acids, such as double stranded DNA that is used in next-generation sequencing (NGS) applications. Nucleic acid-based probes, such as peptide nucleic acid (PNA) oligomers, molecular beacons, DNA flares and locked nucleic acid (LNA) oligomers, are also provided for use in the methods and kits of the present disclosure.

The present invention relates to a modified PNA oligomer for detecting gene methylation. By using a PNA probe modified by introducing a methyl group-specific substituent to the gamma position, N-terminus or C-terminus of the PNA, the present invention may be used for a method for detecting using a difference in physical properties between a gene and a non-methylated gene caused by an interaction between the probe and methyl groups of the gene.

The present invention relates to a modified PNA oligomer for detecting gene methylation. By using a PNA probe modified by introducing a methyl group-specific substituent to the gamma position, N-terminus or C-terminus of the PNA, the present invention may be used for a method for detecting using a difference in physical properties between a gene and a non-methylated gene caused by an interaction between the probe and methyl groups of the gene.