Provided is a preparation method for a DNA library, comprising a pre-library preparation process, the pre-library preparation process comprising DNA preparation, end repair and 3′ A-tailing, linker connection using an anti-contamination linker, linker connected product purification, pre-library amplification, and amplified pre-library purification. Also provided are a use of the anti-contamination linker in preparing a test kit for DNA library capture, and a method for performing bioinformatic analysis on the DNA library prepared by means of the preparation method of the present invention. The preparation method of the present invention reduces the risk of cross-contamination between samples.

Provided is a preparation method for a DNA library, comprising a pre-library preparation process, the pre-library preparation process comprising DNA preparation, end repair and 3′ A-tailing, linker connection using an anti-contamination linker, linker connected product purification, pre-library amplification, and amplified pre-library purification. Also provided are a use of the anti-contamination linker in preparing a test kit for DNA library capture, and a method for performing bioinformatic analysis on the DNA library prepared by means of the preparation method of the present invention. The preparation method of the present invention reduces the risk of cross-contamination between samples.

The present disclosure describes methods for detecting oligonucleotide presence and/or quantity in a sample.

The present disclosure describes methods for detecting oligonucleotide presence and/or quantity in a sample.

The invention relates to the use of an amine masked moiety in a method of enzymatic nucleic acid synthesis. The invention also relates to said amine masked moieties per se and a process for preparing nucleotide triphosphates comprising said amine masked moieties.

The invention relates to the use of an amine masked moiety in a method of enzymatic nucleic acid synthesis. The invention also relates to said amine masked moieties per se and a process for preparing nucleotide triphosphates comprising said amine masked moieties.

The present invention may provide a small RNA detection sensor comprising: at one end thereof, a first sensing region comprising nucleotides having a sequence complementary to target small RNA; and a PCR-capable region that is coupled to the first sensing region, the small RNA detection sensor to synthesize a replication region complementary to the PCR-capable region by a DNA polymerase by using the target small RNA as a primer, and amplify the PCR-capable region and the replication region.

The present invention may provide a small RNA detection sensor comprising: at one end thereof, a first sensing region comprising nucleotides having a sequence complementary to target small RNA; and a PCR-capable region that is coupled to the first sensing region, the small RNA detection sensor to synthesize a replication region complementary to the PCR-capable region by a DNA polymerase by using the target small RNA as a primer, and amplify the PCR-capable region and the replication region.

The present disclosure provides methods and systems for sequencing nucleic acid molecules in a manner that enables higher sequencing accuracy. Methods and systems provided herein may enable sequences that may have low-accuracy reads, such as homopolymer sequences or other repeating sequences, to be determined at a higher accuracy and efficiency.

The present disclosure provides methods and systems for sequencing nucleic acid molecules in a manner that enables higher sequencing accuracy. Methods and systems provided herein may enable sequences that may have low-accuracy reads, such as homopolymer sequences or other repeating sequences, to be determined at a higher accuracy and efficiency.