The present invention is a method for determining whether a test target group sample is contaminated by a positive control sample during a polymerase chain reaction (PCR) process. A target nucleotide sequence and a unique artificial nucleotide sequence are inserted into a positive control, and a probe for determining a false positive is designed that binds to a partial sequence of the target nucleotide and a partial sequence of the unique artificial nucleotide. According to the present invention, it is possible to simply and accurately determine whether it is a false positive by confirming the presence or absence of a unique artificial nucleotide sequence in the test target group.

The invention provides compositions and methods for simultaneously determining the presence or absence of fetal aneuploidy and the relative amount of fetal nucleic acids in a sample obtained from a pregnant female. The method encompasses the use of sequencing technologies and exploits the occurrence of polymorphisms to provide a streamlined noninvasive process applicable to the practice of prenatal diagnostics.

The invention provides compositions and methods for simultaneously determining the presence or absence of fetal aneuploidy and the relative amount of fetal nucleic acids in a sample obtained from a pregnant female. The method encompasses the use of sequencing technologies and exploits the occurrence of polymorphisms to provide a streamlined noninvasive process applicable to the practice of prenatal diagnostics.

Kits for detecting analyte polynucleotides and an internal control in a sample. Included in the kit are an internal control polynucleotide and amplification reagents to co-amplify a first analyte polynucleotide and the internal control. Also included are first and second hybridization probes, each having a label indistinguishable from the other. The probes are respectively capable of hybridizing with a first analyte amplicon and an internal control amplicon. The first and second labels are indistinguishable homogeneous labels.

Kits for detecting analyte polynucleotides and an internal control in a sample. Included in the kit are an internal control polynucleotide and amplification reagents to co-amplify a first analyte polynucleotide and the internal control. Also included are first and second hybridization probes, each having a label indistinguishable from the other. The probes are respectively capable of hybridizing with a first analyte amplicon and an internal control amplicon. The first and second labels are indistinguishable homogeneous labels.

The present invention relates to a set of references nucleic acids for use in a method of detecting methylated CpG-containing nucleic acids by nucleic acid amplification and preferably melting curve analysis of amplification products.

The present invention relates to a set of references nucleic acids for use in a method of detecting methylated CpG-containing nucleic acids by nucleic acid amplification and preferably melting curve analysis of amplification products.

Provided herein are direct-to-library methods, systems, and compositions.

Provided herein are direct-to-library methods, systems, and compositions.

Disclosed herein include systems, methods, compositions, and kits for determining the presence of a single cell mRNA sequencing assay workflow failure, including determining a failure in barcoding copies of a nucleic acid target and determining a failure in sequencing library generation. There are also provided, in some embodiments, compositions, methods, and systems for determining the sequencing status of sequencing library members (e.g., saturated sequencing or under sequencing). Compositions comprising a predetermined copy number of barcoded control nucleic acids are also provided herein.