The present disclosure provides methods and systems for sequencing nucleic acid molecules using a single frequency during detection, or fewer frequencies than types of nucleotide bases identified during detection. Methods and systems of the present disclosure may involve transiently binding nucleotides. Methods and systems provided herein may enable sequences to be determined at a higher accuracy and efficiency.

The present disclosure provides methods and systems for sequencing nucleic acid molecules using a single frequency during detection, or fewer frequencies than types of nucleotide bases identified during detection. Methods and systems of the present disclosure may involve transiently binding nucleotides. Methods and systems provided herein may enable sequences to be determined at a higher accuracy and efficiency.

A composition for detecting SARS-CoV-2 is provided; moreover, a kit including the composition, the use of the kit, and a method for detecting SARS-CoV-2 are also provided.

A composition for detecting SARS-CoV-2 is provided; moreover, a kit including the composition, the use of the kit, and a method for detecting SARS-CoV-2 are also provided.

A detection method including bringing liquid including a target nucleic acid into contact with a well array having wells such that at most one molecule of the target nucleic acid is included per well, sealing the well such that the target nucleic acid remains in the well, amplifying, in the well, a signal derived from the target nucleic acid, detecting the signal emitted from the well, and detecting whether the target nucleic acid includes at least one of a first nucleic acid, a second nucleic acid, and a double-stranded nucleic acid resulting from complementary binding of the first and second nucleic acids. The signal includes at least one of a first signal emitted by binding of a first specific binding substance to the first nucleic acid, and a second signal different from the first signal and emitted by binding of a second specific binding substance to the second nucleic acid.

A detection method including bringing liquid including a target nucleic acid into contact with a well array having wells such that at most one molecule of the target nucleic acid is included per well, sealing the well such that the target nucleic acid remains in the well, amplifying, in the well, a signal derived from the target nucleic acid, detecting the signal emitted from the well, and detecting whether the target nucleic acid includes at least one of a first nucleic acid, a second nucleic acid, and a double-stranded nucleic acid resulting from complementary binding of the first and second nucleic acids. The signal includes at least one of a first signal emitted by binding of a first specific binding substance to the first nucleic acid, and a second signal different from the first signal and emitted by binding of a second specific binding substance to the second nucleic acid.

The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci.

The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci.

The present invention provides a method of determining integrity and/or quantity of cell free DNA (cfDNA) in a biological sample comprising amplifying target sequences with at least a first primer/probe set and at least a second primer probe/set, amplifying the target sequences of differing lengths, and monitoring for detection of the labels of the oligonucleotide probes, and determining the integrity and/or quantity of the cfDNA based on the level of detection of the label of the oligonucleotide probe from the first primer/probe set compared to the level detection of the label of the oligonucleotide probe from the second primer/probe set. The present invention also provides methods for generating a library with the cfDNA for sequencing and analysis.

The present invention provides a method of determining integrity and/or quantity of cell free DNA (cfDNA) in a biological sample comprising amplifying target sequences with at least a first primer/probe set and at least a second primer probe/set, amplifying the target sequences of differing lengths, and monitoring for detection of the labels of the oligonucleotide probes, and determining the integrity and/or quantity of the cfDNA based on the level of detection of the label of the oligonucleotide probe from the first primer/probe set compared to the level detection of the label of the oligonucleotide probe from the second primer/probe set. The present invention also provides methods for generating a library with the cfDNA for sequencing and analysis.