Provided in the present invention is a composition capable of detecting and typing novel coronavirus 2019-nCoV, coronavirus 229E, coronavirus NL63, coronavirus OC43, coronavirus HKU1, coronavirus MERSr-CoV, and coronavirus SARSr-CoV. At the same time, further provided is a kit comprising the composition and a method for detecting and typing coronaviruses. The composition of the present invention in combination with a fluorescent probe method and a melting curve method can perform simultaneous detection and typing of seven coronaviruses in one tube.

Disclosed herein are antigenic peptide-MHC complexes, termed comPACT polypeptides and comPACT polynucleotides, and methods of producing such complexes. Also discloses herein are methods of producing libraries of comPACT polynucleotides and polypeptides, and their exemplary use in capturing cancer neoepitope-reactive T cells with high accuracy. Dual particle detection approaches for detection of neoantigen specific T cells with improved sensitivity and specificity are provided. Signal to noise ratio analysis of isolated T cells for detection of neoantigen-specific T cells with improved T cells is also provided.

Disclosed herein are antigenic peptide-MHC complexes, termed comPACT polypeptides and comPACT polynucleotides, and methods of producing such complexes. Also discloses herein are methods of producing libraries of comPACT polynucleotides and polypeptides, and their exemplary use in capturing cancer neoepitope-reactive T cells with high accuracy. Dual particle detection approaches for detection of neoantigen specific T cells with improved sensitivity and specificity are provided. Signal to noise ratio analysis of isolated T cells for detection of neoantigen-specific T cells with improved T cells is also provided.

Kit, probe mixture and probes for the detection of a chromosome aberration. Genetic probe, obtained by a method comprising the following steps: (a) Examine genomic segments for sequence regions with non-repetitive nucleic acid sequences and select one or more nucleic acid sequences; (b) Design and synthesize primer pairs for a polymerase chain reaction on the non-repetitive nucleic acid sequences, where the primers each have an oligonucleotide sequence which is complementary to the strand or complementary strand of the non-repetitive nucleic acid sequence, and a non-complementary universal linker sequence; (c) Carry out a first PCR and obtain a first mixture (pool A) of nucleic acid fragments; (d) Carry out a multiplex PCR on the mixture (pool A) with the use of primers which hybridize onto the linkers, and obtain a mixture (pool B) with amplified, non-repetitive nucleic acid fragments which are suitable for chromogenic or fluorescence in-situ hybridization of chromosomes (FISH/CISH/ISH).

Kit, probe mixture and probes for the detection of a chromosome aberration. Genetic probe, obtained by a method comprising the following steps: (a) Examine genomic segments for sequence regions with non-repetitive nucleic acid sequences and select one or more nucleic acid sequences; (b) Design and synthesize primer pairs for a polymerase chain reaction on the non-repetitive nucleic acid sequences, where the primers each have an oligonucleotide sequence which is complementary to the strand or complementary strand of the non-repetitive nucleic acid sequence, and a non-complementary universal linker sequence; (c) Carry out a first PCR and obtain a first mixture (pool A) of nucleic acid fragments; (d) Carry out a multiplex PCR on the mixture (pool A) with the use of primers which hybridize onto the linkers, and obtain a mixture (pool B) with amplified, non-repetitive nucleic acid fragments which are suitable for chromogenic or fluorescence in-situ hybridization of chromosomes (FISH/CISH/ISH).

This disclosure provides methods, compositions and kits for determining if nucleic acids detected in a sample such as a clinical sample are derived from contaminant pathogens or clinically-relevant pathogens.

Compositions, kits and methods for detecting a plurality of targets are provided herein. A probe-set composition is provided, including one or more first probes and one or more second probes. Each of the first probe includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a first label, and a cleavage site for a first cleavage agent, wherein the first cleavage agent is capable of releasing the first label. Each of the second probes includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a second label, a quench moiety that renders the second label undetectable, and a cleavage site for the first cleavage agent. The first cleavage agent is capable of releasing the quench moiety, whereby the second label is rendered detectable.

Compositions, kits and methods for detecting a plurality of targets are provided herein. A probe-set composition is provided, including one or more first probes and one or more second probes. Each of the first probe includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a first label, and a cleavage site for a first cleavage agent, wherein the first cleavage agent is capable of releasing the first label. Each of the second probes includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a second label, a quench moiety that renders the second label undetectable, and a cleavage site for the first cleavage agent. The first cleavage agent is capable of releasing the quench moiety, whereby the second label is rendered detectable.

The present disclosure relates to new compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

The present disclosure relates to new compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.