The present invention provides a paper-based, nucleic acid-detecting sensor capable of easily and simply detecting the presence of a target nucleic acid from a PCR amplicon. In addition, the present invention provides a paper-based, nucleic acid-detecting kit capable of easily and simply detecting the presence of a target nucleic acid from a PCR amplicon and a nucleic acid detecting method using same. The present invention can easily and simply determine the presence or absence of a target nucleic acid in a PCR amplicon by utilizing the function in which the target nucleic acid is associated with nanoparticles to form a composite and when loaded into the sensor, the composite is separated and moves according to the structure of the sensor and is finally visualized on the sensor.

Diagnostic devices for performing diagnostic tests are provided, as well as methods that utilize the diagnostic devices, methods for manufacturing the diagnostic devices, and test kits for performing the diagnostic tests. The diagnostic devices may include a sample chamber; a fluid chamber connected to the sample chamber by a conduit; and a test chamber separated from the sample chamber by a breakable seal. A movable liner may form a portion of an inner surface of the sample chamber. The sample chamber may be configured to receive a sample swab, which may move in a first direction when being inserted in the sample chamber to an inserted position, and which may to move in a second direction to cause the breakable seal to break. Movement of the sample swab in the second direction may cause the liner to move in the second direction to break the breakable seal.

Diagnostic devices for performing diagnostic tests are provided, as well as methods that utilize the diagnostic devices, methods for manufacturing the diagnostic devices, and test kits for performing the diagnostic tests. The diagnostic devices may include a sample chamber; a fluid chamber connected to the sample chamber by a conduit; and a test chamber separated from the sample chamber by a breakable seal. A movable liner may form a portion of an inner surface of the sample chamber. The sample chamber may be configured to receive a sample swab, which may move in a first direction when being inserted in the sample chamber to an inserted position, and which may to move in a second direction to cause the breakable seal to break. Movement of the sample swab in the second direction may cause the liner to move in the second direction to break the breakable seal.

A method of using a Defined Swab for genetic identification of microbes, said method comprising contacting said swab with a target to absorb a sample of said target; extracting said sample from said swab; and microbiologically testing said sample to identify said microbe.

A method of using a Defined Swab for genetic identification of microbes, said method comprising contacting said swab with a target to absorb a sample of said target; extracting said sample from said swab; and microbiologically testing said sample to identify said microbe.

The invention provides methods for detecting specific nucleic acids using a loop-mediated isothermal amplification (LAMP) reaction and providing a digital readout of the results. One method teaches separating target specificity from indicator detection in a two-stage multiple-threshold array (MTA). During the first stage amplicons incorporating both target sequence and an indicator sequence are synthesized. During the second stage of the reaction, the indicator sequence is targeted and amplified to produce visual results that may be digitalized. Another method teaches a competitive MTA, in which a reference sequence is used to compete against target sequence during amplification producing threshold responses, thus enabling digital readout. Using either method, quantitation is achieved without the need for continuous monitoring of the reaction and the end-point readout is amenable to visual inspection. Methods of the invention are especially useful in circumstances where there is lower or intermittent power supply.

The invention provides methods for detecting specific nucleic acids using a loop-mediated isothermal amplification (LAMP) reaction and providing a digital readout of the results. One method teaches separating target specificity from indicator detection in a two-stage multiple-threshold array (MTA). During the first stage amplicons incorporating both target sequence and an indicator sequence are synthesized. During the second stage of the reaction, the indicator sequence is targeted and amplified to produce visual results that may be digitalized. Another method teaches a competitive MTA, in which a reference sequence is used to compete against target sequence during amplification producing threshold responses, thus enabling digital readout. Using either method, quantitation is achieved without the need for continuous monitoring of the reaction and the end-point readout is amenable to visual inspection. Methods of the invention are especially useful in circumstances where there is lower or intermittent power supply.

The present application relates to a biochemical reaction test strip tube and a use method thereof, and a kit. The biochemical reaction test strip tube includes a tube portion and a cover portion. The tube portion includes: a tube body including a first chamber and a second chamber; a limit unit provided at an outer side of the tube body; and a biochemical chromatographic test strip provided inside the second chamber, where a first end of the biochemical chromatographic test strip is provided with a sample absorption pad. In this structure, different chambers are provided in the closed test tube, such that a biochemical reaction solution in one chamber is directly detected on the biochemical chromatographic test strip in another chamber, which avoids the solution leakage; and different chambers are provided at different heights, such that the chambers holding biochemical reaction reagents can be selectively heated.

The present application relates to a biochemical reaction test strip tube and a use method thereof, and a kit. The biochemical reaction test strip tube includes a tube portion and a cover portion. The tube portion includes: a tube body including a first chamber and a second chamber; a limit unit provided at an outer side of the tube body; and a biochemical chromatographic test strip provided inside the second chamber, where a first end of the biochemical chromatographic test strip is provided with a sample absorption pad. In this structure, different chambers are provided in the closed test tube, such that a biochemical reaction solution in one chamber is directly detected on the biochemical chromatographic test strip in another chamber, which avoids the solution leakage; and different chambers are provided at different heights, such that the chambers holding biochemical reaction reagents can be selectively heated.

Systems and methods for rapid diagnostics related to the use of CRISPR effector systems and optimized guide sequences, including multiplex lateral flow diagnostic devices and methods of use, are provided.