Compositions comprising microbiological strains and cultures and methods of use thereof are provided herein. Certain strains, cultures, and compositions thereof are useful for the control of head blight disease, for example, of various crop plants. Biological control compositions, and methods of use thereof to prevent, inhibit or treat the development of plant pathogens or disease and for preserving plant yield, are also provided.

The present invention provides methods to improve the health and vigor, including enhancement of the growth of plants, including important crop plants. In particular, the present invention provides methods of treating or preventing a rhizogenic or tumorigenic plant disease caused by bacteria, comprising administering to the plant an effective amount of at least one isolated Paenibacillus strain or of an extract of at least one isolated Paenibacillus strain, wherein said at least one Paenibacillus strain comprises a 16S rRNA sequence with at least 93% sequence identity to the sequence of SEQ ID NO: 1.

The present invention relates to Bacillus methylotrophicus strain DR-08 which produces a natural volatile compound and has antimicrobial activity, and a use thereof. The Bacillus methylotrophicus strain DR-08 according to the present invention, which is an antagonistic microorganism for controlling phytopathogenic microorganisms, and secondary metabolites produced by the strain, which include antibacterially- and antifungally-active non-volatile and volatile materials, are harmless to humans and domestic animals, and exhibit excellent control activity against various phytopathogenic bacteria and fungi while not causing environmental pollution. Thus, the strain and the secondary metabolites can be very usefully used for controlling various plant diseases in an environmentally-friendly manner.

Methods of controlling a plant pathogen, e.g. fungal and bacterial pathogens, on a plant, are described wherein an agricultural composition comprising Bacillus amyloliquefaciens FCC1256 deposited as ATCC No. PTA-122162, a carrier, a surface-active agent and optionally a buffer, and a corresponding concentrate is also described.

A method for preparing a universal microbial medium by eutectic system-based cellulose liquefaction. The method includes: 1) mixing ionic liquid and cellulose, where the ionic liquid is preheated; 2) mixing a mixed solution I obtained in step 1) and distilled water; 3) filtering a mixed solution II obtained in step 2); and 4) diluting a filtrate obtained in step 3) with the distilled water to obtain the carbon source. A medium prepared from the carbon source can be used for culturing various microorganisms, with a desirable growth state of the microorganisms.

The present disclosure is generally related to compositions and methods for constructing and obtaining Bacillus sp. host cells (strains) having enhanced protein production phenotypes. Certain embodiments of the disclosure are therefore related to genetically modified Bacillus sp. cells derived (obtained) from parental Bacillus sp. cells (e.g., such as parental Bacillus cells expressing/producing a protein of interest). Thus, certain embodiments are directed to modified Bacillus sp. cells expressing/producing an endogenous protein of interest, or a heterologous protein of interest, wherein the modified Bacillus sp. cell comprises a genetic modification of a yitMOP operon, a combined genetic modification of a yitMOP operon and a sdpABC operon, a genetic modification of a yitM gene and the like, wherein the modified Bacillus sp. cells produce an increased amount of the POI relative the parental cells from which they were derived (i.e., when grown/cultivated/fermented under identical conditions).

The present invention relates to compositions and methods for treating or preventing gastroesophageal reflux disease (GERD) and related conditions. The subject invention utilizes a composition comprising digestive enzymes, one or more probiotic bacteria, and microbe-based products that can be administered to a subject in need thereof.

An object of the present invention is to provide a novel culturing method by which spores can be efficiently produced. The present invention further provides a method for culturing sporulating bacteria, comprising adding a sporulation-inhibiting substance into a medium for culturing sporulating bacteria, wherein the carbon content in the medium is 9.1 g/L or more, and preferably further comprising a step of adding a sporulation-accelerating substance to the medium.

The present invention provides a biological microbial treating agent, for radioactive material removal, comprising at least one type of microorganism selected from Bacillus amyloliquefaciens KS-R01, Bacillus siamensis KS-R02, Bacillus velezensis KS-R03 and Bacillus tequilensis KS-R04.

A Bacillus altitudinis ST15 antagonizing Xanthomonas oryzae and use thereof, the Bacillus altitudinis ST15 was deposited in China General Microbiological Culture Collection Center on Jun. 28, 2020 and has the deposit number of CGMCC No. 20156. The Bacillus altitudinis has a relatively high antagonistic activity to Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola, can promote growth of rice and improve a drought resistance of a rice seedling, and has a relatively strong tolerance to common chemical microbicides for controlling bacterial diseases of rice. A bacterial suspension or a biocontrol inoculant prepared from this strain has a relatively high biocontrol effect on bacterial blight of rice and bacterial leaf streak of rice, can replace or reduce chemical pesticides, improves safety of food and ecological environment, and has relatively high economic and social benefits.