Provided are biologically pure bacterial isolates characterized by a genome structure at least 90% similar to a genome structure of a bacterial species selected from the group consisting of: Streptomyces sp. E128 having an NRRL Accession No. B-67462, Bacillus amyloliquefaciens A190 having an NRRL Accession No. B-67464, Bacillus subtilis P243 having an NRRL Accession No. B-67459, Bacillus thuringiensis M979 having an NRRL Accession No. B-67457, Massilia aurea P63 having an NRRL Accession No. B-67461, Rhodococcus sp. G706, Stenotrophomonas maltophilia E132 having an NRRL Accession No. B-67460, Streptomyces aurantiacus A918, Streptomyces badius O180, Streptomyces mirabilis B670 having an NRRL Accession No. B67463, Streptomyces scopuliridis F427 having an NRRL Accession No. B-67458, and Streptomyces sp. L219. Also provided are whole cell broth or lysates thereof, and polynucleotide, polypeptides and constructs expressing same, compositions-of-matter comprising same and methods using same for killing or inhibiting the development of insects.

The present application discloses method of controlling plant pathogen(s), e.g. fungal and bacterial pathogens, on a plant, wherein a composition comprising Bacillus amyloliquefaciens FCC1256 deposited as ATCC No. PTA-122162 is applied to the plant, in particular to over-ground parts of the plant. The composition may comprise iturin and fengycin in a relative weight ratio of 1.3:1.0 to 3.0:1.0. The application also discloses an agricultural composition comprising the novel strain, a carrier, a surface-active agent and optionally a buffer, and a corresponding concentrate.

The present disclosure provides a microbial agent YF, including four strains: Bacillus sonorensis B-2, Bacillus paralicheniformis B-26, Bacillus sonorensis N-16, and Bacillus pumilus N-17. The four strains were all deposited in the China General Microbiological Culture Collection Center (CGMCC) on Mar. 5, 2020. The Bacillus sonorensis B-2 has a deposit accession number of CGMCC NO. 19454, the Bacillus paralicheniformis B-26 has a deposit accession number of CGMCC NO. 19449, the Bacillus sonorensis N-16 has a deposit accession number of CGMCC NO. 19448, and the Bacillus pumilus N-17 has a deposit accession number of CGMCC NO. 19450. The microbial agent provided by the present disclosure can effectively prevent stem rot of corn in saline-alkali land, improve the quality of saline-alkali land, and significantly promote the growth of corn.

An object is to obtain a β-amylase having heat resistance even to high temperatures exceeding 60° C. By screening β-amylase producing bacteria from a soil sample, a novel Bacillus halosaccharovorans strain having a novel β-amylase with heat resistance and the β-amylase from the novel B. halosaccharovorans strain were obtained.

The disclosure provides a method of using a bacterium that is a Bacillus, Streptomyces, Microbacterium, Micrococcus, Rhodococcus, Pseudomonas, Arthrobacter or Staphylococcus and/or a biosurfactant-containing extract isolated from said bacterium, as an antimicrobial agent against a foodborne or a plant bacterial or fungal pathogen, wherein the bacterium optionally comprises at least 3 of bacilysin, difficidin, macrolactin h, bacillaene, bacillomycin d, fengycin, surfactin and bacillibactin. The disclosure also provides a plant or plant part bacterized or coated with a Bacillus or a biosurfactant-containing extract isolated from the Bacillus and a method of protecting a plant or plant part against a bacterial or fungal pathogen comprising bacterizing or coating the plant or plant part with a Bacillus or a biosurfactant-containing extract isolated from the Bacillus, wherein the Bacillus produces or the extract contains at least 3 of bacilysin, difficidin, macrolactin h, bacillaene, bacillomycin d, fengycin, surfactin and bacillibactin.

The present invention discloses a Bacillus methylotrophicus strain named Bacillus methylotrophicus BP1.1, which was deposited in China Center for Type Culture Collection under Deposit No. CCTCC M 20191078 on Dec. 20, 2019. The present invention further discloses the use of the Bacillus methylotrophicus strain for degrading benzophenone ultraviolet sunscreens. By domesticating the activated sludge of the domestic sewage treatment plant step-by-step, the present invention provides a Bacillus methylotrophicus BP1.1 strain which has high efficiency in removing benzophenone ultraviolet sunscreens in water environment.

The present invention discloses an equal energy deformation composite foundation using microorganism to solidify aggregate and a construction method thereof, the composite foundation comprises a pile body and a cushion layer, wherein the pile body is provided with several piles, the cushion layer is arranged at the top of the pile body, the pile body is connected into an integral structure through the cushion layer, and the pile body and the cushion layer are filled with aggregate solidified by microorganism. The method comprises the following steps: step 1, leveling the site; Step 2, construction preparation; Step 3, the pile driver in place; Step 4, forming a hole by hammering; Step 5, filling aggregate into the hole; Step 6, repeating the work of step 5; Step 7, forming an equal energy deformation compaction pile using microorganism to solidify aggregate; Step 8, moving to the next pile; Step 9, tamping the ground; Step 10, until the cushion is flush with the surface. Beneficial effects: using local materials, turning waste into wealth, being environmental friendly, saving project cost and conforming to the concept of green development.

An isolated bacterial strain Bacillus amyloliquefaciens 298 is provided. A sample of the strain having been deposited as KCTC 13469BP at the Korean Collection for Type Cultures or a functional homolog of same wherein said isolated microbial strain is purified to a level of at least 99%. Also provided are compositions and articles comprising the bacterial strain and methods of using same.

The present disclosure relates to the isolation, identification and characterization of novel sporeforming probiotic strain(s) with NSPase (Non-Starch Polysaccharides-active hydrolases) activity isolated from fish gut microbiota, methods and uses thereof. The sporeforming probiotic strain(s) with NSPase activity now disclosed are able of producing carbohydrate-active enzymes (CAZymes) that hydrolyse non-starch polysaccharides (NSPs) and accesses their potential as probiotics (PRO) for use in particular in aquafeeds.

A traditional Chinese medicine polysaccharide extract freeze-drying protective agent, a direct-to-vat starter and preparation methods thereof are disclosed, which belong to the technical field of feed starter. The compound traditional Chinese medicine polysaccharide extract freeze-drying protective agent includes Poria cocos polysaccharide extract, Atractylodes macrocephala polysaccharide extract, Radix codonopsis polysaccharide extract, L-tyrosine, α-cyclodextrin and polyethyleneimine. Its application in the freeze-drying protection of direct-to-vat starter of aquatic fermentation feed can reduce the damage to the cells caused by freeze-drying, improve the survival rate of the cells in the starter after freeze-drying, effectively prolong the storage period of the starter, and ensure that the fermentation activity remains at a high level during storage.