A novel method is used for the fermentative production of L-lysine using bacteria of the species Corynebacterium glutamicum, having the ability to excrete L-lysine, containing in their chromosome a variant of a polynucleotide encoding a polypeptide having hydrolase activity. The bacteria are cultivated in a suitable medium under suitable conditions, and then L-lysine is accumulated in the medium to form an L-lysine containing fermentation broth.

The present disclosure describes methods for generating microbial strains expressing a heterologous bacterial hemoglobin gene that produce biomolecules of interest. In aspects, the disclosure provides novel bacterial strains, which express a heterologous bacterial hemoglobin gene whose expression is controlled by a native Corynebacterium glutamicum promoter or a mutant promoter derived therefrom. Also provided herein are methods for producing a library of bacterial hemoglobin genes using a promoter ladder comprising a plurality of promoters derived from Corynebacterium glutamicum.

Provided is a method for screening a corynebacterium constitutive expression vector promoter on the basis of transcriptome sequencing; and further provided are the corynebacterium constitutive expression vector promoter screened on the basis of transcriptome sequencing, an expression vector comprising the promoter, a recombination strain obtained by transforming a host cell Corynebacterium glutamicum using the expression vector, and applications thereof.

A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.

A novel method is for the fermentative production of L-lysine using bacteria of the species Corynebacterium glulamicum. The bacteria have the ability to excrete L-lysine and contain in their chromosome a polynucleotide, which encodes a polypeptide having the activity of a transcriptional factor. An amino acid at a particular position of the amino acid sequence of the polypeptide has been substituted by a different proteinogenic amino acid.

The present invention provides novel, recombinant Gram-negative bacteria. In particular, the invention provides recombinant Gram-negative bacteria (e.g., E. coli) lacking genes involved in lipopolysaccharide (LPS, endotoxin) biosynthesis (e.g., lacking genes required for core oligosaccharide biosynthesis) and also provides recombinant Gram-negative bacteria lacking genes involved in LPS biosynthesis that contain one or more exogenous KDO transferases and/or one or more exogenous heptosyltransferases (e.g., from one or more types and/or strains of bacteria). The invention further provides methods of generating and utilizing (e.g., as or in an immunogenic composition (e.g., as or in an adjuvant and/or vaccine)) the recombinant Gram-negative bacteria therapeutic, preventative, and/or research applications.

The present application relates to a putrescine-producing microorganism in which the activity of formate dehydrogenase is increased, and a method for producing putrescine using the same.

The present application relates to a novel branched-chain amino acid aminotransferase variant that decreases the amount of by-products generated during the production of L-isoleucine, a polynucleotide encoding the variant, and a vector comprising the polynucleotide, a microorganism comprising the variant, polynucleotide or vector, and a method for producing L-isoleucine using the microorganism.

The present disclosure relates to an isopropylmalate synthase variant and a method of producing L-leucine using the same.

The present application relates to an aroG aldolase (phospho-2-dehydro-3-deoxyheptonate aldolase) variant, a microorganism comprising the same, and a method for producing amino acids using the same.