This invention consists of a consortium of plant-growth promoting microorganisms including Pseudomonas stutzeri, Pseudomonas denitrificans, Pseudomonas resinovorans, Pseudomonas brassicacearum, Pseudomonas fluorescens, Shimwellia blattae and Klebsiella oxytoca. The bacterial consortium along with a vehicle suitable for agricultural application, form a biofertilizer useful to enhance agricultural yield when applied to cultivation plants. The biofertilizer described present a long shelf life and preserve a high cell density along the time.

The present disclosure provides a novel aldehyde synthase gene having activity of synthesizing a medium-chain aldehyde. Such gene encodes a protein comprising the amino acid sequence as shown in SEQ ID NO: 2.

A method for isolating proteins from prokaryotes is disclose. The method enables easy break down of bacterial cell walls to obtain intact proteins without damage by a simple process of adding organic solvents including lower alcohols or nitrile derivatives; or applying osmotic stimulation to samples containing pathogenic bacteria or the like. The method may be usefully applied to rapid and accurate identification of periplasmic proteins of gram-negative bacteria without additional purification process.

The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

The present invention relates to probiotic bacteria of the species Klebsiella oxytoca that are used for a decolonization of multidrug resistant (MDR) Enterobacteria, such as Klebsiella pneumoniae (K. pneumoniae), from the gut of a subject. The decolonization can both be therapeutic, i.e. after colonization of the gut by the multiresistant pathogen(s), or as a preventive measure before a re-colonization of the gut, as required after antibiotic treatment.

To identify a microorganism causing the development of primary sclerosing cholangitis associated with ulcerative colitis. A Klebsiella pneumoniae strain inducing inflammation in the liver.

Disclosed is a method for identifying causative bacteria of sepsis, the method comprising: a step of performing a PCR method using a sample collected from a subject and a combination of sets of primers each specific to the full-length or a partial region of each of gyrB gene of Escherichia coli, nuc gene of Staphylococcus aureus, atlE gene of Staphylococcus epidermidis, gyrB gene of Klebsiella pneumoniae and rpoB gene of Enterococcus spp.; and a step of detecting the presence or absence of amplification of the full-length or the partial region of each of the genes by using a combination of probes each specific to DNA of the full-length or the partial region.

An object of the present disclosure is at least to provide a technique for promoting elimination of a methyl group(s) of a methoxy group(s) in causing a microorganism having a demethylation ability of eliminating a methyl group(s) of a methoxy group(s) from a compound with the methoxy group(s) in a side chain(s) to produce a demethylated compound in which a methyl group(s) of a methoxy group(s) has eliminated from a compound with the methoxy group(s) in a side chain(s). The issue is solved by a method for producing a demethylated compound, comprising co-culturing, in a solution containing a compound with a methoxy group(s) in a side chain(s), a microorganism having a demethylation ability of eliminating a methyl group(s) of a methoxy group(s) from a compound with the methoxy group(s) in a side chain(s), and a microorganism having an activity to promote the demethylation, to produce the demethylated compound in which a methyl group(s) of a methoxy group(s) has eliminated from the compound with the methoxy group(s) in the side chain(s).

The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

Methods and systems are provided for generating and utilizing a bacterial composition that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system that has been fertilized with more than 20 lbs of Nitrogen per acre.