This invention consists of a consortium of plant-growth promoting microorganisms including Pseudomonas stutzeri, Pseudomonas denitrificans, Pseudomonas resinovorans, Pseudomonas brassicacearum, Pseudomonas fluorescens, Shimwellia blattae and Klebsiella oxytoca. The bacterial consortium along with a vehicle suitable for agricultural application, form a biofertilizer useful to enhance agricultural yield when applied to cultivation plants. The biofertilizer described present a long shelf life and preserve a high cell density along the time.

Methods and systems are provided for generating and utilizing a bacterial composition that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system that has been fertilized with more than 20 lbs of Nitrogen per acre.

A system and a method for predicting susceptibility of genus Klebsiella to amikacin are provided. The system includes a calculating unit containing a computer-readable storage medium stored with a computer program; the computer program is used to, when is executed by a processor, implement a calculation method for an Exp(?k) power value, and the calculation method for the Exp(?k) power value includes: step 1, calculating a k value according to formula I; and step 2, calculating the Exp(?k) power value with an Euler's constant e as a base and a ?k value as an exponential; and C1 to C5 are respectively copy numbers of an arr-2 gene, an acrB gene, an armA gene, an oqxB gene, and a rmtB gene in to-be-predicted Klebsiella strains. When the method and the system are adopted to predict the susceptibility of the genus Klebsiella to amikacin, an accuracy rate is about 98.8%.

The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

A system and a method for predicting susceptibility of genus klebsiella to amikacin are provided. The system includes a calculating unit containing a computer-readable storage medium stored with a computer program; the computer program is used to, when is executed by a processor, implement a calculation method for an Exp(?k) power value, and the calculation method for the Exp(?k) power value includes: step 1, calculating a k value according to formula I; and step 2, calculating the Exp(?k) power value with an Euler's constant e as a base and a ?k value as an exponential; and C1 to C5 are respectively copy numbers of an arr-2 gene, an acrB gene, an armA gene, an oqxB gene, and a rmtB gene in to-be-predicted klebsiella strains. When the method and the system are adopted to predict the susceptibility of the genus klebsiella to amikacin, an accuracy rate is about 98.8%.

The example of the present invention discloses a klebsiella sp. strain and a method for preparing a microbial flocculant from the same. This strain is a Klebsiella sp. strain LDX1-1 which has been preserved in the China General Microbiological Culture Collection Center on Sep. 7, 2015, and the preservation number is CGMCC No. 11330. This strain can be used for preparing a microbial flocculate in an activated fermentation way.

Methods and systems are provided for generating and utilizing a genetically engineered bacterium comprising a modification in a nifA gene or homolog thereof that can result in a bacterium with modified regulation of nitrogen fixation or assimilation activity. Genetically engineered bacteria with modified nitrogen fixation or assimilation activity are also provided. The genetically engineered bacterium can fix nitrogen in the presence of nitrogen (e.g., ammonium), and/or oxygen.

The present invention relates to methods and compositions for treatment of microbial infections and for the enhancement of resistance to infection. The invention comprises administration of an effective amount of a protein isolated from bacterial lysate compositions for the treatment of pathological compositions of microbial infections. The present invention can also be used to enhance the immune system to prevent infections be the administration of an effective amount of compositions.

The present invention relates to methods and compositions for treatment of microbial infections and for the enhancement of resistance to infection. The invention comprises administration of an effective amount of a protein isolated from bacterial lysate compositions for the treatment of pathological compositions of microbial infections. The present invention can also be used to enhance the immune system to prevent infections be the administration of an effective amount of compositions.

Methods and systems are provided for generating and utilizing a genetically engineered bacterium comprising a modification in glnD, wherein said modification is selected from the group consisting of: deletion of the entire gene, deletion of substantially the entire gene, deletion of an ACT domain, deletion of more than 50% of an ACT domain, deactivation of an ACT domain, and deactivation of an UTase domain.