A seed koji (Aspergillus starter) for brewing is provided. The seed koji is obtained by inoculating koji mold(s) belonging to an Aspergillus genus into a seed koji raw material, where the seed koji raw material is a pea.

Provided are methods for identifying surface-exposed epitopes of fungal cell wall proteins, and related peptide antigens, suitable for the development of antifungal antibodies. The methods allow for the detection of surface-exposed epitopes that are particularly highly expressed in drug-resistant fungal pathogens, and in pathogens exposed to antifungal drugs. Also provided are antifungal antibodies which may be derived by these methods. The methods and antibodies find use in the treatment of treating and diagnosing fungal infections, such as candidiasis, aspergillosis cryptococcosis, and Mucormycosis.

Disclosed is a filamentous fungus and use thereof in gas metabolism, which belongs to the technical field of microorganisms. The filamentous fungus is an Aspergillus cejpii strain S8 deposited in the China General Microbiological Culture Collection Center (CGMCC), No. 3, No. 1, West Beichen Road, Chaoyang District, Beijing on Sep. 20, 2023, with a deposit number of CGMCC NO. 40828.

The present invention provides submerged fermentation processes of producing one or more polypeptide of interest, the process comprising: a) fermenting one or more microorganism that produces the one more polypeptide of interest in a fermentation broth, and b) adding at least one enzyme to the fermentation broth: OR co-expressing the at least one enzyme in the one or more microorganism and secreting the at least one enzyme into the fermentation broth in an amount sufficient to reduce the viscosity of the fermentation broth, compared to when the at least one enzyme is not added or co-expressed; and, optionally, c) recovering the one or more polypeptide of interest.

Disclosed is a filamentous fungus and use thereof in gas metabolism, which belongs to the technical field of microorganisms. The filamentous fungus is an Aspergillus cejpii strain S8 deposited in the China General Microbiological Culture Collection Center (CGMCC), No. 3, No. 1, West Beichen Road, Chaoyang District, Beijing on Sep. 20, 2023, with a deposit number of CGMCC NO. 40828.

An application method includes: applying an Aspergillus sp. L14-OE::laeA2 in producing cyclic tripeptides. In the cyclic tripeptides obtained by separating and purifying a fermented product of the Aspergillus sp. L14-OE::laeA2, a yield of aspochracin is 175 mg/L, a yield of JBIR-15 is 100 mg/L, and a yield of sclerotiotide C is 25 mg/L. The yields of the compounds are significantly higher than those reported in other literature, and a separation process of obtaining the compounds is simple and economical. The cyclic tripeptides may be used to prepare drugs with antifungal activity and antioxidants activity, and for inhibiting activity of tumor cells and anti-tumor drugs. At a concentration of 10 M, the JBIR-15 has an inhibition rate of 11.78% on a human hepatocellular carcinoma cell line (Hep-G2), and the sclerotiotide C has an inhibition rate of 21.09% on a human hepatocellular carcinoma cell line (Hep-G2).

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

Disclosed are Aspergillus terreus ANU-301 strain (Accession Number: KACC49929), and ANU-301 strain (Accession Number: KACC49929) and a composition for controlling bacterial plant diseases using the culture filtrate. Since the strain or culture filtrate, according to the present disclosure, enhances the stress resistance of plants, it enables sustainable agriculture to eliminate or minimize the use of chemical pesticides.

The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields, even if an inorganic selenium compound is used as a selenium compound. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant to obtain selenoneine, wherein the transformant has at least one gene selected from the group consisting of a SatA gene, a CysB gene and a MetR gene, and an EgtA gene inserted therein and can overexpress the inserted genes.

An Aspergillus sp. DH4 with aerobic denitrification enhanced by an inorganic electron donor and its application are provided, which relates to the field of microbial technologies. The Aspergillus sp. DH4 is preserved at China Center for Type Culture Collection (CCTCC), a preservation address is Wuhan University, 299 Bayi Road, Wuchang District, Wuhan City, China, a preservation number is CCTCC NO: M20232690, and a preservation date is Dec. 27, 2023.