The present invention relates to a yeast extract having a high tripeptide content and a high dry matter content using a yeast cell containing tripeptide and a method for preparing the same.

The invention discloses a method for preparing the ginsenoside preparation by biological engineering technology, wherein the ginsenoside preparation comprises at least one of the saponin-enriched preparation and the saponin-enriched syrup; the compound bio-enzyme preparation prepared by the combined bacteria preparation of yeast and lactic acid bacteria provided by the invention is rich in amino acid residues, amides, coenzymes and active groups such as lactic acid and linoleic acid; and the ginsenoside preparations containing ginsenosides can be prepared by adding monosaccharides or polysaccharides to the compound bio-enzyme preparations, providing new directions and ideas for obtaining ginsenosides and their products.

Disclosed herein is a method for separating a protein from a microorganism comprising a cell wall. Further compositions comprising a first fraction, a second fraction or both, derived from a microorganism comprising a cell wall and comprising a protein content between 10% and 90% by weight of the fraction, are also disclosed.

Provided is an engineered bacterium for producing nervonic acid and/or grease. The genome of the engineering bacterium is integrated with an expression cassette expressing a protein encoded by 3-ketoacyl-CoA synthase (KCS) gene and/or an exterase gene.

Provided are a recombinant microorganism having a 2,3-dehydroadipyl-CoA reductase activity and a production method of a C6 compound, and are a recombinant microorganism capable of producing adipic acid or an adipic acid derivative, and a production method of adipic acid or an adipic acid derivative.

A recombinant microorganism contains at least one of an exogenous gene encoding a protein having an enzymatic activity to reduce 2,3-dehydroadipyl-CoA for conversion into adipyl-CoA and an exogenous gene encoding a 3-hydroxyadipyl-CoA dehydratase.

The application provides an enzyme immobilization carrier and a preparation method thereof, an immobilized enzyme and a preparation method thereof. The above enzyme immobilization carrier is obtained by an amino modification or a cyanuric chloride modification of super-crosslinked polyvinyl alcohol. The use of the enzyme immobilization carrier provided by the application may effectively improve the stability and reusability of the immobilized enzyme. Moreover, due to the use of the form of enzyme covalent linkage, compared with an embedding method, the preparation method is no need for chemical reagent immersion and the like, it is beneficial to maintain the own activity of the enzyme, and promote the immobilized enzyme to have the better activity while the stability and reusability are kept.

Recombinant yeasts bioengineered to overexpress genes for utilization of fatty acids to produce acetone and isopropanol, and methods of use thereof. The yeasts are modified to express, constitutively express, or overexpress an acetyl-CoA thioesterase, an acetyl-CoA C-acetyltransferase, an acetoacetyl-CoA transferase, an acetoacetyl-CoA thioesterase, an acetoacetate decarboxylase, an isopropanol dehydrogenase, or any combination thereof. The methods include cultivating the recombinant yeasts to convert any fatty acid-containing feedstocks into acetone and/or isopropanol.

Disclosed herein is Candida parapsilosis CGMCC 9630, the carbonyl reductase expressed by said strain and the encoding gene and amino acid sequence thereof, the recombinant expression vector and recombinant expression transformant containing said gene sequence, and use of whole cells of Candida parapsilosis, carbonyl reductase or corresponding recombinant transformant thereof as catalyst in catalyzing asymmetric reduction of prochiral carbonyl compounds, particularly reduction of 6-carbonyl-8-halogenocaprylate to prepare the synthetic precursor of (R)--lipoic acid, (R)-6-hydroxy-8-halogenocaprylate. In comparison to other methods of asymmetric reduction for preparing (R)-6-hydroxy-8-halogenocaprylate, the disclosure has advantages of high substrate concentration, mild reaction conditions, environmental friendship, high yield, and high optical purity of the product, and thus has good prospect in industrial production of (R)---lipoic acid.

Antimicrobial modified defensin or defensin-like peptides, modified C-terminal fragments of a defensin or defensin-like peptides and nucleic acids encoding the same are disclosed. Compositions comprising the defensin variant peptides and methods of their use to control microbial infections of plants and vertebrate subjects as well as contamination of feedstuffs and foodstuffs are also disclosed.

The present invention relates to a method for producing dioic acids from a substrate containing hydrocarbons or fatty acids using a Candida infanticola strain, and to a Candida infanticola microorganism used therefor. The present invention reduces the cost increase resulting from the fluctuation in the international oil price and the burden of environmental pollution, which are caused by the use of fossil fuels, and thus can be utilized in various industrial fields using DDDA as a raw material.