A method for enzymatically preparing a n-3 polyunsaturated fatty acid diacylglycerol using oil rich in n-3 polyunsaturated fatty acid as raw material to prepare diacylglycerol oil with selective enzymatic hydrolysis. Before the enzymatic reaction, the raw materials are homogenized to improve the catalytic efficiency of lipase in the hydrolysis and achieve the purpose of reducing the reaction time and the addition amount of lipase. As a result, the cost of industrial diacylglycerol preparation is reduced, ultimately reducing the cost of industrial diacylglycerol preparation. Therefore, the present invention provides an effective, mild and low-cost enzymatic method for preparing diacylglycerol rich in n-3 polyunsaturated fatty acids, which has a promising potential for industrial application.

Methods and materials related to producing glyceric acid and downstream products are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, methods and materials for producing glycolic acid by direct fermentation from sugars are disclosed.

The present invention relates to a method of genetically modifying a GC rich microorganism. The present invention further relates to a genetically modified GC rich microorganism. Furthermore, the present invention relates to a composition comprising a RNA-guided endonuclease, at least one guide RNA (gRNA), and optionally donor DNA. The present invention also relates to a method of preparing a target compound, e.g. an acetyl-CoA-based hydrophobic compound, and/or an oil having a specific fatty acid profile, e.g. high oleic oil, using a genetically modified GC rich microorganism.

A method for contamination control when growing yeasts is provided. Bacterial contamination is controlled by using urea as the primary nitrogen source while simultaneously limiting the amount of nickel available to contaminating bacteria. Bacteria require nickel as a cofactor for urease enzymes in order to use urea for growth while yeasts do not require nickel as a cofactor for any enzymes. Nickel is limited by using titanium in plate heat exchangers instead of stainless steel. Ethyl carbamate is limited by using a carbon/nitrogen ratio that consumes all urea during fermentation and by separating co-products after fermentation and before distillation. Yeast recycling is performed by using either single-step or two-step centrifugation, without acid washing. This method enables yeast recycling with sugarcane ethanol and sugar beet ethanol production. This method also enables yeast recycling with corn ethanol and grain ethanol production with coproduct recovery after fermentation and before distillation.

The subject invention provides methods for enhanced production of microbial biosurfactants using a fermentation medium that has been sterilized using ozone. The methods provide for higher yields of the biosurfactants, improved color, and reduced energy expenditure compared with standard fermentation techniques that rely upon thermal sterilization.

The invention provides a fungal cell, such as a yeast cell, capable of growing in co-cultivation with Propionibacterium. Also provided are methods of producing such cells and fermentation processes using the fungal cell of the invention and Propionibacterium in co-cultivation. Such co-cultivation significantly reduces the chemical oxygen demand load of the waste fermentation broth.

The present invention belongs to the technical field of biological fermented feeds, in particular to a two-step production method of a mycoprotein biological feed by fermenting corn husks. The present invention provides a preparation method for a mycoprotein biological feed, which comprises: inoculating Aspergillus niger microbial agent on a first fermentation substrate for first fermentation, to obtain a yeast seed; and inoculating a compound microbial agent in a second fermentation substrate to perform second fermentation. The compositions of the first fermentation substrate, the second fermentation substrate and the compound microbial agent are defined. The degradation of cellulose and hemicellulose in the corn husk raw material can be maximized and the protein content is increased through two fermentations, while taking into account the cost. A preparation method of the high-quality protein feed is provided for the feed industry.

Provided are methods for identifying surface-exposed epitopes of fungal cell wall proteins, and related peptide antigens, suitable for the development of antifungal antibodies. The methods allow for the detection of surface-exposed epitopes that are particularly highly expressed in drug-resistant fungal pathogens, and in pathogens exposed to antifungal drugs. Also provided are antifungal antibodies which may be derived by these methods. The methods and antibodies find use in the treatment of treating and diagnosing fungal infections, such as candidiasis, aspergillosis cryptococcosis, and Mucormycosis.

Provided are an immobilized enzyme and an application thereof in continuous production. The immobilized enzyme is a Polyethyleneimine (PEI)-modified immobilized enzyme, and includes: an enzyme, which includes Amine Dehydrogenase (AmDH) and/or Formate Dehydrogenase (FDH); and a carrier, which is a cyanuric chloride-activated amino carrier. The problem in the prior art of poor performance of an immobilized enzyme is solved, the catalytic activity and reusability of the immobilized enzyme are improved, and the immobilized enzyme is suitable for the field of enzyme immobilization.

Provided are: a novel yeast having an ability to efficiently produce ethanol from glucose and xylose in a short time in the coexistence of the glucose and the xylose; and a method for producing ethanol using the novel yeast. A yeast, which was designated as Candida intermedia 4-6-4T2 and was deposited as FERN BP-11509.