Pichia pastoris alcohol dehydrogenase 2 (ADH2) promoter variants include at least one of the specified modifications on wild-type Pichia pastoris ADH2 promoter (SEQ ID NO: 1). The modification includes one of the following mutations: integration of a Cat8 transcription factor binding site (TFBS), particularly integration of SEQ ID NO: 3 or other gene sequences that show at least 80% similarity with this sequence, at any positions within nucleotides a) 647 to 660; b) 739 to 752; c) 1 to 948; and d) mutations specified with SEQ ID NO: 2 within nucleotides 15 to 848 separately and combinations thereof.

Provided herein are recombinant fungal or bacterial cells comprising a heterologous L-lactate dehydrogenase, and processes of preparing L-lactic acid employing such cells.

Provided herein are composition and process for using an electrochemical device for the reduction of the oxidized state of phosphorylated or non-phosphorylated nicotinamide adenine dinucleotide to the reduced state in which unwanted products of the electrochemical reduction are recovered as the oxidized state of the phosphorylated or non-phosphorylated nicotinamide adenine dinucleotide and returned to the electrochemical device for reduction.

Multi-species pest control methods, which contact or subject a target pest to a yeast strain belonging to the species Pichia Kluyveri and/or with a lysate, filtrate, isolate or extract thereof. The yeast strain belonging to the species Pichia Kluyveri and/or the lysate, filtrate, isolate or extract thereof is incorporated into the body of the agricultural target pest.

A fermented sparkling vinegar beverage includes a fermented solution formed of 0 vol % of to 15 vol % of raw vinegar, 3 vol % of to 15 vol % of sugar, 0 vol % of to 5 vol % of honey, 0 vol % of to 0.3 vol % of salt, 0 vol % of to 40 vol % of plant extracts, 1 vol % of to 5 vol % of yeast liquid, and water. Composition of yeast liquid varies according to different product flavors; yeast strains are selected from Saccharomyces cerevisiae, Hanseniaspora osmophila, Hanseniaspora valbyensis, Torulaspora delbrueckii, and Dekkera bruxellensis in different ratios, and the yeast strains generate metabolite and produce bubbles in the fermented solution. Therefore, the present invention brews the fermented solution by yeast liquid formed of various yeasts, applying the metabolites and bubbles generated through fermentation of the yeast strains to alleviate sour content of the raw vinegar, so as to improve the drinking taste of the fermented solution.

The present invention provides a topical composition comprising an extract of Pichia anomala and n-acetyl glucosamine.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

This invention relates to genetically engineered strains of yeast and methods for producing recombinant protein (e.g., collagen). Recombinant protein of the present invention is used to produce biofabricated leather or a material having leather-like properties containing recombinant or engineered collagen. The yeast strains are engineered to produce ascorbate and/or increased production of α ketoglutarate.

The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.