Modified bacterial strains are provided. The strains can generate a desired product such as pyruvate and products derived from pyruvate. Methods of generating pyruvate and products derived from pyruvate are also provided. The modified bacterial strains have at least one mutation in a gene coding for proteins in a pyruvate dehydrogenase complex such that the mutation allows a cell to accumulate pyruvate and/or products derived from pyruvate.

A method for the preparation of 2,4-dihydroxybutyric acid from homoserine includes a first step of conversion of the primary amino group of homoserine to a carbonyl group to obtain 2-oxo-4-hydroxybutyrate, and a second step of reduction of the obtained 2-oxo-4-hydroxybutyrate (OHB) to 2,4-dihydroxybutyrate.

Methods for biosynthesising hydrocarbons from a gaseous substrate in non-naturally occurring acetogens as well as non-naturally occurring acetogens for production of hydrocarbons are provided.

Provided herein are gene editing systems and/or compositions comprising RNA guides targeting LDHA for use in genetic editing of the LDHA gene. Also provide herein are methods of using the gene editing system for introducing edits to the LDHA gene and/or for treatment of primary hyperoxaluria (PH), and processes for characterizing the gene editing system.

Related are a recombinant microorganism for producing L-valine, a construction method and an application thereof. Through transferring an amino acid dehydrogenase gene and/or activating activity of a transhydrogenase and/or a NAD kinase, reducing power of NADPH in cell is increased, the titer and yield of L-valine generated by Escherichia coli are improved, and the production of L-valine by one-step anaerobic fermentation is achieved.

This disclosure relates to oligonucleotides, compositions and methods useful for reducing LDHA expression, particularly in hepatocytes.

A process is described for making acrylic acid from dextrose, which comprises fermenting dextrose; removing solids from the resulting fermentation broth; removing lactic acid from the clarified broth by extraction into an organic solvent; separating out the lactic acid-loaded organic solvent while recycling at least a portion of the remainder back to the fermentation step; reacting the lactic acid with ammonia to provide a dehydration feed comprising ammonium lactate while preferably recycling the organic solvent; carrying out a vapor phase dehydration of the ammonium lactate to produce a crude acrylic acid product; and purifying the crude acrylic acid by distillation followed by melt crystallization, chromatography or both melt crystallization and chromatography.

The present disclosure relates to genetically engineered methanotrophic bacteria with the capability of growing on a multi-carbon substrate as a primary or sole carbon source and methods for growing methanotrophic bacteria on a multi-carbon substrate.

Provided is a transformant which can produce lactic acid with a high productivity without requiring neutralization with an alkali and is excellent in both of lactic acid production capability and growth ability and its production process, and a method for producing lactic acid by using the transformant.

A transformant containing from 3 to 5 copies of a lactate dehydrogenase gene derived from human and introduced into a Schizosaccharomyces pombe host, wherein a gene that is a part of a group of genes encoding pyruvate decarboxylase of the Schizosaccharomyces pombe host is deleted or inactivated. A transformant characterized by providing a cell concentration of at least 4.0 g (on a dry cell weight basis)/L of a culture broth prepared by inoculating cells, at an initial cell concentration of 0.04 g (on a dry cell weight basis)/L, into a 500 mL Sakaguchi flask containing 100 mL of a liquid culture broth which includes 1% of yeast extract, 2% of peptone and 6% of glucose, and then cultivating 20 hours at a temperature of 32° C. under shaking conditions of 110 rpm and 7 cm stroke, and providing a lactic acid concentration of at least 80 g/L of a fermentation liquor prepared by inoculating cells obtained by cultivating 20 hours under the above-described conditions, at an initial cell concentration of 36 g (on a dry cell weight basis)/L, into a 11.1% glucose aqueous solution, and then fermenting 3 hours at a temperature of 32° C. under shaking conditions of 110 rpm and 7 cm stroke. A method for producing lactic acid by using these transformants.

The present invention relates to: a novel Pichia kudriavzevii microorganism NG7 showing heat resistance and acid resistance; a composition, for producing organic acid or alcohol, which comprises the microorganism and a culture of the same; and a method, for producing an organic acid or alcohol, which comprises culturing the microorganism.