A molecular manufacturing process includes contacting a platform molecule with (i) a biocatalyst and (ii) a chemical catalyst under conditions suitable to produce a value-added chemical.

The present invention relates to biocatalytic methods, comprising purely enzymatic, mixed enzymatic-fermentative and purely fermentative methods, for the direct single-step conversion of L-fucitol to L-fucose, in order to easily obtain L-fucose at high amounts and levels of purity. Suitable recombinant microorganisms and fungi are further disclosed and also the use thereof in said method for the single-step conversion to L-fucose.

A chemoenzymatic method of producing a polyhydroxybenzene includes contacting glucose with one or more biocatalysts under conditions suitable for the formation of D-glucodialdose; and thermally treating D-glucodialdose in the presence of a dehydration catalyst under conditions suitable for the formation of the polyhydroxybenzene. A chemoenzymatic method of producing 1,2,3,4-tetrahydroxybenzene includes contacting glucose with a mutated copper radical oxidase and catalase under conditions suitable for the formation of D-glucodialdose; and subjecting D-glucodialdose and a dehydration catalyst to temperatures ranging from about 40 C. to about 100 C. suitable for the formation of 1,2,3,4-tetrahydroxybenzene.

The present invention provides engineered galactose oxidase (GOase) enzymes, polypeptides having GOase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing GOase enzymes are also provided. The present invention further provides compositions comprising the GOase enzymes and methods of using the engineered GOase enzymes. The present invention finds particular use in the production of pharmaceutical and other compounds.

Disclosed are modified galactose oxidase variants with enhanced protein expression, solubility, thermal stability, and enzymatic activity, and reduced enantioselectivity compared to wild-type galactose oxidase or galactose oxidase variants known in the art, and methods of producing the same.

The present invention provides engineered galactose oxidase (GOase) enzymes, polypeptides having GOase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing GOase enzymes are also provided. The present invention further provides compositions comprising the GOase enzymes and methods of using the engineered GOase enzymes. The present invention finds particular use in the production of pharmaceutical and other compounds.

A chemoenzymatic manufacturing process for the preparation of high value chemicals includes the enzymatic oxidation of ethanol using an oxidizing biocatalyst to form an acetaldehyde intermediate. In addition, the process includes contacting of the acetaldehyde intermediate with a carboligating biocatalyst to form pyruvic acid. Further, the process includes reacting pyruvic acid with ethanol to form ethyl pyruvate. Still further, the process includes contacting the ethyl pyruvate with a metal catalyst and hydrogen to form ethyl lactate. The process also includes hydrolyzing the ethyl lactate to produce lactic acid. Moreover, the process includes contacting at least a portion of the lactic acid with a dehydrating catalyst to form acrylic acid. In addition, the process includes contacting at least a portion of the lactic acid with a metal catalyst and hydrogen to form propylene glycol and n-propanol.