A starch biosynthesis method may implement total artificial biosynthesis from simple compounds such as dihydroxyacetone, formaldehyde, formic acid and methanol to starch. By coupling with methods such as chemical reduction of carbon dioxide, even total artificial biosynthesis of starch taking carbon dioxide as a starting raw material can be implemented. The method can utilize carbon dioxide of high concentration and high density and electric energy and hydrogen energy of high energy density, is more suitable for an industrial production mode, and the production cycle is shortened from several months in farming to several days.

The present invention relates to a recombinant yeast cell for high yield protein expression. The invention further relates to cell culture involving the recombinant yeast cell, a method for preparing protein involving culturing the recombinant yeast cell and a use of the recombinant yeast cell.

The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate. The organism can further contain a hydrogen synthesis pathway. The invention additionally provides methods of using such microbial organisms to produce butadiene or 2,4-pentadienoate by culturing a non-naturally occurring microbial organism containing butadiene or 2,4-pentadienoate pathways as described herein under conditions and for a sufficient period of time to produce butadiene or 2,4-pentadienoate. Hydrogen can be produced together with the production of butadiene or 2,4-pentadienoate.

This document relates to materials and methods for the production of protein. In one aspect, this document provides a nucleic acid construct including a first alcohol oxidase promoter element, wherein the first alcohol oxidase promoter element includes a mutation at one or more nucleotide positions corresponding to any of nucleotide positions 668-734 relative to SEQ ID NO: 28.

The use of UV-activated Copper Radical Oxidase (CRO) enzymes in the implementation of oxidation reactions. Also, a process for oxidizing organic compounds using enzymes which are activated by UV light. The process also leads to concomitant formation of hydrogen peroxide, that can optionally be used in hydrogen peroxide mediated processes. Further, the process relates to the oxidation of alcohols in aldehydes.

A method including the process steps of insertion of a gene to be expressed into the plasmid carrying AOX1 promoter (i), cloning of plasmid carrying gene (ii), transfer of recombinant plasmid carrying AOX1 promoter and gene to expression host (iii) and providing both induction of AOX1 promoter and expression of associated heterologous gene, using a nitric oxide donor (iv). The method enables expression of various genes of various microorganisms, plants, animals and human, and thus extracellular and/or intracellular production of various recombinant proteins.

Maintaining functionality in a device reliant on active biocatalysts. A water-impermeable container has one or more biocatalysts mixed and packed with stabilizers to function as a slow-release device. Depending on its adjustable geometries the device will provide a modifiable trickle of biocatalysts into a liquid released from a dry and stable state.

The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides viable bacterial cells which comprise heterologous dual 3-hydroxy-acyl-ACP dehydratase/isomerases, etc. The disclosure further provides monounsaturated fatty acid derivative molecules produced by the viable bacterial cells which are non-native to the bacterial cells. The disclosure further provides methods for the preparation and production of non-native monounsaturated fatty acid derivative molecules such as e.g., an ω3-monounsaturated fatty acid derivative, an ω5-monounsaturated fatty acid derivative, an ω9-monounsaturated fatty acid derivative, an ω11-monounsaturated fatty acid fatty acid derivative, etc.

A process for the enzymatic oxidation of sulfinic acids includes sulfinic acids of formula H.sub.2NCH(R)CH.sub.2SO.sub.2H to sulfonic acids of formula H.sub.2NCH(R)CH.sub.2SO.sub.3H and an enzyme selected from the class of H.sub.2O.sub.2-generating oxidases in the presence of the substrate of said enzyme.

Provided herein are compositions with enhanced protein content, proteins with high solubility, protein combinations and methods for the preparation thereof.