The present invention relates to improved methods for producing a hydrophobic compound, in particular a hydrophobic compound which is a pheromone such as an insect pheromone, in a fermentation process involving cultivation of a microorganism such as a yeast, said microorganism producing said hydrophobic compound, wherein the methods facilitate recovery of the hydrophobic compound from the fermentation broth, increase the titer of the hydrophobic compound and/or increase secretion of the hydrophobic compound from the microorganism.

The present invention relates to a process for the production of methyl methacrylate. The process of the present invention comprises the steps of: a) providing a microorganism in a fermentation medium, under conditions which said microorganism will produce a C.sub.3-C.sub.12 methacrylate ester; b) providing an organic phase in contact with the fermentation medium, said organic phase including C.sub.3-C.sub.12 methacrylate ester in a higher concentration than that in the fermentation medium; c) removing organic phase containing the said C.sub.3-C.sub.12 methacrylate ester from contact with the fermentation medium; and d) transesterifying the removed C.sub.3-C.sub.12 methacrylate ester with methanol, optionally after separation from the organic phase, to produce methyl methacrylate.

The present invention relates to a process for the production of methyl methacrylate. The process of the present invention comprises the steps of: a) providing a microorganism in a fermentation medium, under conditions which said microorganism will produce a C.sub.3-C.sub.12 methacrylate ester; b) providing an organic phase in contact with the fermentation medium, said organic phase including C.sub.3-C.sub.12 methacrylate ester in a higher concentration than that in the fermentation medium; c) removing organic phase containing the said C.sub.3-C.sub.12 methacrylate ester from contact with the fermentation medium; and d) transesterifying the removed C.sub.3-C.sub.12 methacrylate ester with methanol, optionally after separation from the organic phase, to produce methyl methacrylate.

The present invention relates to a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain, to the preparation of a long-chain dibasic acid producing strain by directed evolution of POX gene and homologous recombination, and to the production of a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain by using the strain. The present invention also relates to a strain containing a mutated promoter, wherein, when a long-chain dibasic acid is produced by fermentation of this strain, the content of the acid impurity of shorter carbon chain in the fermentation product is significantly reduced.

The present invention relates to a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain, to the preparation of a long-chain dibasic acid producing strain by directed evolution of POX gene and homologous recombination, and to the production of a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain by using the strain. The present invention also relates to a strain containing a mutated promoter, wherein, when a long-chain dibasic acid is produced by fermentation of this strain, the content of the acid impurity of shorter carbon chain in the fermentation product is significantly reduced.

The present invention relates to a method for producing dioic acids from a substrate containing hydrocarbons or fatty acids using a Candida infanticola strain, and to a Candida infanticola microorganism used therefor. The present invention reduces the cost increase resulting from the fluctuation in the international oil price and the burden of environmental pollution, which are caused by the use of fossil fuels, and thus can be utilized in various industrial fields using DDDA as a raw material.

The invention relates to various new yeast strains of the type Yarrowia lipolytica as well as relevant methods for the biocatalytic preparation of ?-hydroxy fatty acids or dicarboxylic acids with the aid of these strains, whereby the formation of ?-hydroxy fatty acids or dicarboxylic acids is advantageously increased.

The present invention relates to a method for producing a medium chain diamine and, more specifically, to a method for producing a medium chain diamine from an alcohol or alkane derived from a fatty acid, by culturing a recombinant microorganism from which a fatty aldehyde dehydrogenase gene in a -oxidative metabolic pathway and a -oxidative metabolic pathway related gene have been deleted, and also into which a -transaminase gene has been introduced. The recombinant microorganism disclosed in the present invention can prevent the additional oxidation and -oxidation metabolism of fatty aldehyde and can produce a medium chain diamine with a high yield by introducing an amine group to the terminus thereof.

The invention relates to various new yeast strains of the type Yarrowia lipolytica as well as relevant methods for the biocatalytic preparation of ?-hydroxy fatty acids or dicarboxylic acids with the aid of these strains, whereby the formation of ?-hydroxy fatty acids or dicarboxylic acids is advantageously increased.

The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production. Provided are engineered microorganisms capable of producing fatty dicarboxylic acids and products expressed by such microorganisms. Also provided are biological methods for producing fatty dicarboxylic acids.