The present invention relates to a recombinant nucleic acid molecule, a recombinant microorganism, to a method for producing alanine and to the use of the recombinant nucleic acid molecule or the recombinant microorganism for the fermentative production of alanine.

The present invention relates to a recombinant nucleic acid molecule, a recombinant microorganism, to a method for producing alanine and to the use of the recombinant nucleic acid molecule or the recombinant microorganism for the fermentative production of alanine.

Surprisingly, it has been found that alkaliphilic bacteria of the genus Corynebacterium are naturally suited to produce L-amino acids.

A microorganism produces guanidinoacetic acid (GAA) and has at least one gene coding for a protein having the function of a NADH-dependent dehydrogenase. A method for the fermentative production of GAA uses such microorganism. A method produces creatine through fermentative production. Industrial feed stocks are used as starting material in the fermentative process.

The present invention discloses a DL-alanine-producing genetically engineered strain, as well as a method of construction and use thereof, and pertains to the field of bioengineering. According to the present invention, through enhancing the glycolysis pathway or/and introducing thermostable alanine dehydrogenase, a genetically engineered strain capable of high-yield production of alanine at 42 C. to 55 C. This strain can be used in a two-step method for producing racemic DL-alanine, which includes fermentation and subsequent addition of microbial alanine racemase. Through inactivating or deleting alanine racemase genes in this strain and then separately introducing overexpressed alanine racemase gene(s), a genetically engineered strain capable of producing racemic DL-alanine using a direct fermentation method can be constructed. When the original strain possesses a lactate synthesis pathway, blocking this lactate synthesis pathway in both the genetically engineered strains can additionally augment the proportion of a pyruvate synthesis pathway.

This disclosure provides methods and genetically engineered strains of Vibrio natriegens, specifically developed for the bio-based production of succinate. Capitalizing on the rapid growth kinetics and highly efficient carbon metabolism of V. natriegens, this disclosure provides an environmentally friendly, scalable, and cost-effective alternative to traditional petrochemical methods for succinate production.

The present invention discloses an L-alanine-producing genetically engineered strain, as well as a method of construction and use thereof, and pertains to the field of bioengineering. According to the present invention, through enhancing the glycolysis pathway or/and introducing a gene for thermostable alanine dehydrogenase, a genetically engineered strain capable of high-yield production of alanine under a high temperature condition of 42 C. to 55 C. can be constructed. Moreover, through knocking out alanine racemase genes, optical purity of L-alanine can be significantly increased. When the original strain possesses a lactate synthesis pathway, blocking this lactate synthesis pathway can augment the proportion of a pyruvate synthesis pathway, resulting in an additionally increased yield of L-alanine. The present invention overcomes the problems of fermentation at a low temperature, high cost and the like, which arise from the use of conventional L-alanine production techniques, enables production of L-alanine by fermentation at a high temperature of 42 C. to 55 C. with a yield of 95 g/L or higher, and is of high value to industrial application.

The present invention relates to a cell capable of producing a fluorinated compound, in particular F-acetaldehyde and optionally F-acetyl-CoA and F-acetate, methods for producing fluorinated compounds in a cell and expression systems thereof.

Compositions and methods for the preparation of immobilized enzymes for use in biocatalytic processes are provided. More specifically, the immobilized enzyme systems are covalently cross-linked biomolecular condensates that stabilize the enzymes without impeding their effectiveness as biocatalyst.