This application relates to laccase variants and uses thereof as eco-friendly biocatalysts in various industrial processes. More in particular, the disclosure relates to a polypeptide with laccase activity comprising an amino acid sequence that is at least 75% identical to the amino acid sequence according to SEQ ID NO: 1, wherein the polypeptide comprises an amino acid residue selected from the group consisting of threonine, proline, alanine, glycine, serine, cysteine, aspartic acid, asparagine and valine at a position corresponding to amino acid 149 of SEQ ID NO: 1.

This application relates to laccase variants and uses thereof as eco-friendly biocatalysts in various industrial processes. More in particular, the disclosure relates to a polypeptide with laccase activity comprising an amino acid sequence that is more than 80% identical to the amino acid sequence according to SEQ ID NO: 1, wherein the polypeptide comprises a non-polar amino acid, preferably an amino acid residue selected from the group consisting of proline, alanine, glycine and valine at a position corresponding to amino acid 113 of SEQ ID NO: 1.

The present application is directed to lettuce plants comprising a mutation in each of at least two different PPO genes, where said mutations reduce the activity of PPO protein compared to a wild type lettuce plant. The present application is also directed to methods for making such lettuce plants.

The present invention relates to recombinant bacteria to which a novel lipid peroxidation system has been applied and a method for preparing a biofuel and a biopolymer using same. The artificial lipid peroxidation system of the present invention can be used to prepare ultra-high concentrations of free fatty acids through artificial lipid peroxidation without cell death, and a biofuel and a biopolymer can be prepared therefrom. In addition, the artificial lipid peroxidation system according to the present invention increases intracellular redox energy density and can thus additionally promote carbon dioxide fixation. Accordingly, it is possible to prepare increased quantities of a biofuel and a biopolymer.

The present invention relates to a process for clarifying a liquid obtained from a plant material, wherein said process involves the use of laccase and fungal -glucans.

The invention provides an enzyme granule comprising a mixture of a laccase and a buffer in an amount capable of maintaining an alkaline pH.

The object of the present invention is to provide a processing method that makes it possible to improve various characteristics related to a fermented food or beverage that uses a protein ingredient (and, in particular, characteristics of the fermented food or beverage itself such as stress, water retention, suppression of water release, and the like or characteristics of the ingredients of the fermented food or beverage related to fermentation speed during production of the fermented food or beverage). The present invention improves various characteristics of a fermented food or beverage that uses a protein ingredient using the action of a protein deamidase and a multicopper oxidase during production of the fermented food or beverage.

Disclosed are methods and apparatuses utilizing an O.sub.2-insensitive FDH2 from the sulfate-reducing bacterium (SRB) Desulfovibrio vulgaris Hildenborough (DvH). The O.sub.2-insensitive FDH2 may be applied to a biofuel cell for generating electricity and generating hydrogen peroxide. The biofuel cell can also be applied to wearable or implantable devices as a power source. The O.sub.2-insensitive FDH2 can also be used in other applications not applying a fuel cell, such as hydrogen peroxide generation, a formate testing kit, or carbon capture applications.

Various aspects of this disclosure relate to the discovery that phosphatase and laccase enzymes confound the extraction and quantification of psilocybin and other tryptamines during the preparation of psilocybin-containing products from mushrooms. Various aspects of this disclosure relate to the discovery that ionization states of phosphoryloxytryptamines affect the extraction and quantification of psilocybin and other tryptamine. This disclosure describes methods to denature enzymes, optimize ionization states, and control for other variables to improve extraction yields of tryptamines from mushrooms, manufacturing intermediates, and tryptamine-containing products and to improve the accuracy of methods to quantify tryptamines.

Various aspects of this disclosure relate to the discovery that phosphatase and laccase enzymes confound the extraction and quantification of psilocybin and other tryptamines during the preparation of psilocybin-containing products from mushrooms. Various aspects of this disclosure relate to the discovery that ionization states of phosphoryloxytryptamines affect the extraction and quantification of psilocybin and other tryptamine. This disclosure describes methods to denature enzymes, optimize ionization states, and control for other variables to improve extraction yields of tryptamines from mushrooms, manufacturing intermediates, and tryptamine-containing products and to improve the accuracy of methods to quantify tryptamines.