Patent classifications
C
CHEMISTRY; METALLURGY
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C12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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C12Y
ENZYMES
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114/00
Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
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C12Y114/13
with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
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C12Y114/13121
Premnaspirodiene oxygenase (1.14.13.121)
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C12Y114/13121

STABILIZATION OF CYTOCHROME P450 REDUCTASE
20170044556 · 2017-02-16 ·

The present invention relates to a recombinant microorganism which is capable of expressing a cytochrome P450 reductase (CPR) and, optionally, a cytochrome P450 enzyme (CYP) and which is capable of overexpressing a Ice2p comprising the amino acid sequence set out in SEQ ID NO: 2 or a sequence having at least 50% sequence identity thereto. The invention further relates to use of Ice2p to stabilize expression of a cytochrome P450 reductase in a recombinant microorganism.