An object of the present invention is to provide DNA of a glycoalkaloid biosynthetic enzyme of a solanaceous plant (Solanaceae) such as a potato. The present invention relates to a protein having glycoalkaloid biosynthetic enzyme activity of a solanaceous plant such as a potato and a method for producing/detecting a novel organism using a gene encoding the protein.

The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C (CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.

Methods of inhibiting cytochrome P450 2D6 enzymes are provided that can be used for improving the treatment of diseases by preventing degradation of drugs or other molecules by cytochrome P450 2D6. Pharmaceutical compositions are provided that can act as boosters to improve the pharmacokinetics, enhance the bioavailability, and enhance the therapeutic effect of drugs that undergo in vivo degradation by cytochrome P450 2D6 enzymes.

Described herein are biological devices and extracts useful for detecting alcohol and/or opioids in a biological sample from a subject; the biological devices and extracts can also be useful for detoxifying alcohol and/or opioids in a subject in need thereof. The biological devices include microbial cells transformed with a DNA construct containing genes for producing sulfotransferase, UDP-glucuronosyltransferase, O-linked N-acetylglucosamine transferase, alcohol dehydrogenase, and cytochrome P450. In some instances, the biological devices also include a gene for enhanced green fluorescent protein. Methods for using the devices are also provided herein.

An object of the present invention is to provide DNA of a glycoalkaloid biosynthetic enzyme of a solanaceous plant (Solanaceae) such as a potato. The present invention relates to a protein having glycoalkaloid biosynthetic enzyme activity of a solanaceous plant such as a potato and a method for producing/detecting a novel organism using a gene encoding the protein.

Provided is a composition for producing astringin among metabolites of polydatin, wherein the astringin may be mass-produced by oxidizing the polydatin using a CYP102A1 chimera and mutants thereof as a catalyst, the CYP102A1 chimera being produced by fusing a reductase domain of a wild-type CYP102A1 which is a bacterial cytochrome P450 enzyme, with a heme domain of a CYP102A1 mutant.

The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons.

This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.

Described herein are CB5 polypeptides for the biosynthesis of oxygenated hydrocarbons in modified host cells.

The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.