This disclosure describes transgenic plants engineered for enhanced amounts of fatty acids and triacylglycerols (TAGs) in vegetative tissues. This document describes novel approaches to develop biomass crops, such as Sorghum, for production of vegetative sources of vegetable oils in leaves and stems as alternative renewable diesel and sustainable aviation fuel (SAF) feedstocks. A new approach is described for engineering high vegetative oil production that involves expression of variant medium-chain fatty acid acyl-acyl carrier protein (AGP) thioesterases to drive vegetable oil production. This approach has been shown to be effective with two different acyl-ACP thioesterases, and the high oil production is maintained over multiple genetic generations in greenhouse and field cultivation systems.

The present invention relates to microbial cells comprising triacylglycerol (TAG) with short chain fatty acids (SCFA), as well as methods of using these cells to produce lipid comprising TAG with SCFAs.

The subject invention pertains to environmentally-friendly compositions, methods and systems for the production of diesel biofuels. More specifically, the subject invention integrates electrocatalysis and microbial bioconversion to generate lipids from carbon dioxide in a way that increases energy efficiency over known biomass-derived diesel production and significantly reduces land use.