A nucleotide sequence is shown in SEQ ID NO.1. The gene encodes mannosyltransferase I. The gene plays an important role in the normal reproductive development of the Nilaparvata lugens (Stål). Inhibition of the function of the gene may reduce the survival rate of the Nilaparvata lugens (Stål) and hinder embryonic development. With respect to reduction of the survival rate of the Nilaparvata lugens (Stål) and hindering of embryonic development, the present invention can reduce the harm of the Nilaparvata lugens (Stål) to rice by killing the Nilaparvata lugens (Stål). By using the characteristic that the nucleotide sequence of a highly conserved target gene has no homology with the nucleotide sequence of natural enemies of the Nilaparvata lugens (Stål), RNA interference is performed at a nucleic acid level, to avoid the harm to non-target organisms such as natural enemies, thereby realizing green control of the Nilaparvata lugens (Stål) while controlling pests.

A nucleotide sequence is shown in SEQ ID NO.1. The gene encodes mannosyltransferase I. The gene plays an important role in the normal reproductive development of the Nilaparvata lugens (St?l). Inhibition of the function of the gene may reduce the survival rate of the Nilaparvata lugens (St?l) and hinder embryonic development. With respect to reduction of the survival rate of the Nilaparvata lugens (St?l) and hindering of embryonic development, the present invention can reduce the harm of the Nilaparvata lugens (St?l) to rice by killing the Nilaparvata lugens (St?l). By using the characteristic that the nucleotide sequence of a highly conserved target gene has no homology with the nucleotide sequence of natural enemies of the Nilaparvata lugens (St?l), RNA interference is performed at a nucleic acid level, to avoid the harm to non-target organisms such as natural enemies, thereby realizing green control of the Nilaparvata lugens (St?l) while controlling pests.

The present invention relates to a cell-free enzyme-catalyzed process for producing glycoproteins of general formula (I) from a lipid-linked oligosaccharide and a peptide. Further, said process includes the construction of the lipid-linked oligosaccharide from a mannose trisaccharide containing core structure. Particularly, the lipid-linked oligosaccharide is a high mannose-, complex-, or hybrid-type N-glycan.