The present invention relates to compounds, compositions, and methods are provided for covalently linking a cargo molecule, such as a therapeutic or a diagnostic agent, to a glycan in the Fab region of an antibody. Also provided are methods of modeling and producing antibodies having de novo Fab glycosylation sites. Also provided are antibody carrier conjugates, methods of using the conjugates.

A mammalian or avian cell line that expresses high levels of human influenza virus receptors is provided. In one embodiment, the cell line supports human influenza virus, e.g., human A/H3 influenza virus, isolation and growth much more effectively than corresponding conventional (unmodified) cells or in corresponding human virus receptor-overexpressing cells, and the propagated viruses may maintain higher genetic stability than in the corresponding cells.

This disclosure relates to glycoengineering, and methods of utilizing glycoengineering for various therapeutic purposes.

Glycoproteins having particular sialylation patterns, and methods of making and using such glycoproteins, are described.

A bender includes a rotatable bending shoe having at least one channel therein configured to receive a workpiece, a seat proximate to the bending shoe which is configured to receive the workpiece thereon, and a sensor provided on the seat which is configured to sense the presence of the workpiece. In some embodiments, the sensor is configured to sense an end of the workpiece.

The invention relates to a microbial production of recombinant oligosaccharides, particularly of human milk oligosaccharides (HMOs), using a genetically modified microorganism, particularly E. coli, in antibiotic-free cultivating medium.

2-6-Sialyltransferase (2,6ST) variants having improved 2-6-specific sialidase activity as compared to the native 2,6ST enzymes are described. The variants include GT80 sialyltransferases such as P. damselae Pd2,6ST. Methods for making de-sialylated products and screening sialidase activity are also described.

The present invention relates to cell lines that are genetically modified to overexpress a -galactoside -2,3-sialyltransferase 1 (ST3Gal1), preferably human ST3Gal1, which can be used for the production of recombinant glycoproteins having highly or fully sialylated O-linked GalNAc glycans (GalNAc O-glycans), preferably core 1 GalNAc O-glycans, as well as to respective recombinant glycoproteins. Further, the present invention relates to respective methods of expressing recombinant glycoproteins, methods of increasing the degree of sialylation of recombinant glycoproteins, and methods of decreasing the micro-heterogeneity of GalNAc O-glycans. Finally, the present invention relates to respective uses of the above cell lines for the production of recombinant glycoproteins, for increasing the degree of sialylation of recombinant glycoproteins, and for decreasing the micro-heterogeneity of O-linked GalNAc glycans of recombinant glycoproteins.

A bender includes a rotatable bending shoe having a plurality of channels therein configured to receive a workpiece and a follower assembly. The follower assembly includes a mount, a follower bar attached to the mount, the follower bar having a plurality of channels therein configured to receive the workpiece, a roller attached to the mount and engaged against the follower bar, the roller having an eccentric shaft extending therefrom, and a motor for rotating the shaft and roller, wherein rotation of the shaft and roller causes the follower bar to translate toward or away from the bending shoe. A drive member can be provided to move the mount toward or away from the bending shoe.

A mammalian or avian cell line that expresses high levels of human influenza virus receptors is provided. In one embodiment, the cell line supports human influenza virus, e.g., human A/H3 influenza virus, isolation and growth much more effectively than corresponding conventional (unmodified) cells or in corresponding human virus receptor-overexpressing cells, and the propagated viruses may maintain higher genetic stability than in the corresponding cells.