The present disclosure describes systems and methods capable of fixing atmospheric nitrogen into bio-available nitrogenous compounds, including ammonia. Embodiments of the present disclosure are directed to synthetic DNA constructs encoding genes to allow release of bio-available nitrogenous compounds in nitrogen fixing diazotrophic organisms. Many of these constructs encode these genes in inducible and constitutive means, such that inducible embodiments can be activated at select times. Additional embodiments are directed to genetically engineered diazotrophs utilizing these constructs to produce bio-available nitrogenous compounds. Further embodiments are directed to methods to create these constructs and organisms as well as to use these constructs and organisms.

The present disclosure describes systems and methods capable of fixing atmospheric nitrogen into bio-available nitrogenous compounds, including ammonia. Embodiments of the present disclosure are directed to synthetic DNA constructs encoding genes to allow release of bio-available nitrogenous compounds in nitrogen fixing diazotrophic organisms. Many of these constructs encode these genes in inducible and constitutive means, such that inducible embodiments can be activated at select times. Additional embodiments are directed to genetically engineered diazotrophs utilizing these constructs to produce bio-available nitrogenous compounds. Further embodiments are directed to methods to create these constructs and organisms as well as to use these constructs and organisms.