The present invention relates to methods for controlling hybridization in plants and producing hybrid plants. The present invention also relates to nucleic acids encoding amino acid sequences for self-incompatibility (SI) proteins in plants, and the use thereof for the manipulation of SI, including seed production, in plants, particularly of the Poaceae family. The present invention also relates to kits, compositions, constructs and vectors including such nucleic acids, and related polypeptides, regulatory elements and methods. The present invention also relates to expression of self-gamete recognition genes in plants and to related nucleic acids, constructs, molecular markers and methods.

A new turfgrass APMT 008 (ATCC Accession No. PTA-126635). A method of over-seeding dormant warm season grasses, including over-seeding the warm season grass with Turf Type Festulolium (American Ryegrass) such that the American Ryegrass will provide a green cover during the winter months but will die out when the temperatures increase to the point which allow the warm-season grass to regrow. A method to maintain green coverage of turf year-round including planting a warm season grass, and over-seeding the warm season grass with American Ryegrass. The method where the American Ryegrass is selected from the group consisting of breeder's codes: APMT001, APMT002, APMT003, APMT004, APMT005, APMT006, APMT007, APMT008, APMT009, APMT010, and APMT011.

A new turfgrass APMT 005 (ATCC Accession No. PTA-126634). A method of over-seeding dormant warm season grasses, including over-seeding the warm season grass with Turf Type Festulolium (American Ryegrass) such that the American Ryegrass will provide a green cover during the winter months but will die out when the temperatures increase to the point which allow the warm-season grass to regrow. A method to maintain green coverage of turf year-round including planting a warm season grass, and over-seeding the warm season grass with American Ryegrass. The method where the American Ryegrass is APMT005.

A method for producing haploid Lolium plants may start with providing a Lolium multiflorum inducer line, the L. multiflorum inducer line having the ability to induce mitotic genome instability and haploid sectoring when hybridized as a maternal parent with a Lolium sp. paternal parent, such as previously disclosed lines IL1 and IL2. The inducer line may then be crossed with a Lolium sp. to generate F1 plants, and the F1 plants may be self-fertilized so as to recover seed from the selfed plant. The recovered seed may then be planted to generate one or more F2 plants, and at least one of the F2 plants may be a haploid Lolium plant.

Dihaploid recoveries of Schendonorus meadow fescue and festulolium may be created using the method disclosed herein. The method includes crossing a ryegrass (Lolium multiflorum) inducer line (IL) with individuals from a meadow fescue or festulolium cultivar or population. Such a cross can create a variety of resulting individual plants, including dihaploid recoveries. Some of the dihaploid recoveries can also be rhizomatous even when the parental meadow fescue or festulolium plant did not display a rhizomatous trait.

A method of over-seeding dormant warm season grasses, including over-seeding the warm season grass with Turf Type Festulolium (American Ryegrass) such that the American Ryegrass will provide a green cover during the winter months but will die out when the temperatures increase to the point which allow the warm-season grass to regrow. A method maintain green coverage of turf year-round including planting a warm season grass, and over-seeding the warm season grass with American Ryegrass. The method where the American Ryegrass is selected from the group consisting of breeder's codes: APMT001, APMT002, APMT003, APMT004, APMT005, APMT006, APMT007, APMT008, APMT009, APMT010, and APMT011.

A method of over-seeding dormant warm season grasses, including over-seeding the warm season grass with Turf Type Festulolium (American Ryegrass) such that the American Ryegrass will provide a green cover during the winter months but will die out when the temperatures increase to the point which allow the warm-season grass to regrow. A method for maintaining green coverage of turf year-round including planting a warm season grass, and over-seeding the warm season grass with American Ryegrass. The method where the American Ryegrass is selected from the group consisting of breeder's codes: APMT001, APMT002, APMT003, APMT004, APMT005, APMT006, APMT007, APMT008, APMT009, APMT010, and APMT011.

The tall fescue DH66OP Accession No. PI 690020 represents a genetic stock possessing an L. multiflorum cytoplasm that can enhance or induce rhizome formation across various genotypes. DH66OP may allow for the transfer the L. multiflorum cytoplasm to additional tall fescue genotypes for the recovery of genotypes expressing rhizomes.

A method of over-seeding dormant warm season grasses, including over-seeding the warm season grass with Turf Type Festulolium (American Ryegrass) such that the American Ryegrass will provide a green cover during the winter months but will die out when the temperatures increase to the point which allow the warm-season grass to regrow. A method maintain green coverage of turf year-round including planting a warm season grass, and over-seeding the warm season grass with American Ryegrass. The method where the American Ryegrass is selected from the group consisting of breeder's codes: APMT001, APMT002, APMT003, APMT004, APMT005, APMT006, APMT007, APMT008, APMT009, APMT010, and APMT011.

A method for producing haploid Lolium plants may start with providing a Lolium multiflorum inducer line, the L. multiflorum inducer line having the ability to induce mitotic genome instability and haploid sectoring when hybridized as a maternal parent with a Lolium sp. paternal parent, such as previously disclosed lines IL1 and IL2. The inducer line may then be crossed with a Lolium sp. to generate F1 plants, and the F1 plants may be self-fertilized so as to recover seed from the selfed plant. The recovered seed may then be planted to generate one or more F2 plants, and at least one of the F2 plants may be a haploid Lolium plant.