The disclosure provides a method of generating a sterile fish, crustacean, or mollusk. The method comprises breeding (i) a fertile hemizygous mutated female fish, crustacean, or mollusk with (ii) a fertile hemizygous mutated male fish, crustacean, or mollusk, selecting a female progenitor that is homozygous by genotypic selection, and breeding the homozygous female progenitor to produce the sterile fish, crustacean, or mollusk. The mutation disrupts the maternal-effect of a primordial germ cell (PGC) development gene and does not impair the viability, sex determination, fertility, or a combination thereof, of a homozygous progenitor. The disclosure also provides methods of making broodstock freshwater and seawater organisms for use in producing sterilized freshwater and seawater organisms, as well as the broodstock itself.

The invention provides a light generating system for arthropod keeping, configured to generate system light, wherein in a first operational mode the light generating system is configured to provide the system light having a spectral power distribution, wherein the spectral power distribution comprises: a first spectral power E.sub.1 in a first wavelength range of 360-780 nm; a second spectral power E.sub.2 in a second wavelength range of 360-400 nm; a third spectral power E.sub.S in a third wavelength range of 400-480 nm; a fourth spectral power E.sub.M in a fourth wavelength range of 480-580 nm; a fifth spectral power E.sub.L in a fifth wavelength range of 580-700 nm; a sixth spectral power E.sub.6 in a sixth wavelength range of 620-700 nm; a seventh spectral power E.sub.7 in a seventh wavelength range of 700-780 nm; and wherein: 1.75≤E.sub.M/E.sub.S≤20; E.sub.2/E.sub.1≤0.005; E.sub.7/E.sub.1≤0.022; and E.sub.L/E.sub.1≤0.3; or 0.3<E.sub.L/E.sub.1≤0.8, and 3.4≤E.sub.6/E.sub.S≤14, and wherein the sixth wavelength range comprises a peak between 650-690 nm.

The present invention provides a means for regulating (promoting or suppressing) the growth of Decapoda crustaceans such as shrimps and crabs. Methods of the present invention regulate the growth of animals belonging to the Decapoda, comprising a step of regulating (inhibiting or enhancing) the function of genes comprising: at least one growth regulation-related gene selected from the group consisting of mTOR pathway, Akt pathway, and upstream and downstream factors of the pathways; and further optionally at least one molting-related gene selected from a molting-related factor or the function of a transcription or translation product of the genes.

The present invention provides systems and non-invasive methods for gender selection of eukaryotic organisms More specifically, the invention applies the CRISPR-Cas system as well as any derivatives and fusion proteins thereof for creation of transgenic eukaryotic organisms and for selecting the desired gender of the resulting progeny.

This disclosure describes exemplary embodiments of a method of creating a YY animal broodstock, preferably in a single generation, wherein the broodstock includes only sperm-producing YY males and egg-producing YY males, the method comprising the steps of: (a) creating YY males via androgenesis; (b) exposing selected ones of the YY males created in step (a) to a feminizing hormone; and (c) identifying sperm-producing YY males and egg-producing YY males from among the YY males created in steps (a) and (b). In other embodiments, the method further comprises: (d) repeating steps (a) through (c) N times in order to produce N unrelated families of sperm-producing YY males and egg-producing YY males; and (e) cross-breeding various ones of the unrelated families produced in step (d) in order to produce a genetically-diverse YY progeny. In some embodiments, N may be about 60.

This disclosure describes exemplary embodiments of a method of creating a YY animal broodstock, preferably in a single generation, wherein the broodstock includes only sperm-producing YY males and egg-producing YY males, the method comprising the steps of: (a) creating YY males via androgenesis; (b) exposing selected ones of the YY males created in step (a) to a feminizing hormone; and (c) identifying sperm-producing YY males and egg-producing YY males from among the YY males created in steps (a) and (b). In other embodiments, the method further comprises: (d) repeating steps (a) through (c) N times in order to produce N unrelated families of sperm-producing YY males and egg-producing YY males; and (e) cross-breeding various ones of the unrelated families produced in step (d) in order to produce a genetically-diverse YY progeny. In some embodiments, N may be about 60.

The present invention provides a method for editing the genome of at least one cell of a crustacean embryo, the method including contacting a crustacean embryo or a cell thereof with an effective amount of a gene editing agent. Further provided is a crustacean including at least one cell comprising an edited genome, obtained according to the method of the invention.

The invention provides a light generating system for arthropod keeping, configured to generate system light, wherein in a first operational mode the light generating system is configured to provide the system light having a spectral power distribution, wherein the spectral power distribution comprises: a first spectral power E.sub.1 in a first wavelength range of 360-780 nm; a second spectral power E.sub.2 in a second wavelength range of 360-400 nm; a third spectral power E.sub.S in a third wavelength range of 400-480 nm; a fourth spectral power E.sub.M in a fourth wavelength range of 480-580 nm; a fifth spectral power E.sub.L in a fifth wavelength range of 580-700 nm; a sixth spectral power E.sub.6 in a sixth wavelength range of 620-700 nm; a seventh spectral power E.sub.7 in a seventh wavelength range of 700-780 nm; and wherein: 1.75E.sub.M/E.sub.S20; E.sub.2/E.sub.10.005; E.sub.7/E.sub.10.022; and E.sub.L/E.sub.10.3; or 0.3<E.sub.L/E.sub.10.8, and 3.4E.sub.6/E.sub.S14, and wherein the sixth wavelength range comprises a peak between 650-690 nm.