The present disclosure provides a novel gene drive methodology that can be used to spread engineered traits into a targeted population. More specifically, the present disclosure provides a method of using a transgenic line containing a modified Cas9, e.g., nickase Cas9, that introduces single-strand breaks/nicks, instead of double-stranded DNA cuts, to promote super-Mendelian inheritance of an engineered gene drive allele. The present disclosure further provides a Cx.quin. Cas9 (e.g., nickase Cas9) line and a Cx.quin. gene-drive line for suppression populations.

A configuration system for a green energy agricultural park includes a mushroom cultivation section (10), a super-intensive breeding section (20), a non-toxic vegetable planting section (30), a poultry breeding section (40), a CAS cold chain section (50), a black soldier fly breeding section (60), a goods collection sales section (70), an electric vehicle charging section (80), and a green energy power generation section (90). The green energy power generation section generates electricity and supplies an electric power to the mushroom cultivation section, the super-intensive breeding section, the non-toxic vegetable planting section, the poultry breeding section, the CAS cold chain section, the black soldier fly breeding section, the goods collection sales section, and the electric vehicle charging section. The black soldier fly breeding section breeds black soldier flies to eat agricultural organic resources produced in the green energy agricultural park and to generate black soldier fly manure.

Embodiments provided herein relate to systems for synthetically-engineered reciprocal chromosomal translocation for gene insertion into a population of organisms such as insects.

Embodiments provided herein relate to systems for synthetically-engineered reciprocal chromosomal translocation for gene insertion into a population of organisms such as insects.

Provided is a gene in drosophila designated as dSLC5A1. This gene is responsible for taste independent nutrient sensing. Activation of neurons expressing this gene results in hunger behavior. Provided are compositions and methods for identifying agents that can interfere with hunger behavior.

Reagents and methods are provided that allow for an improved expression of a recombinant protein in an insect, More specifically, the introduction of recombinant DNA elements into an insect larva allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in the survival rate after infection of the insect These recombinant DNA elements can be introduced, for example, into insect larvae via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional, enhancer elements, such as the homologous region (hr) and promoters.

A method of enhancing resistance of a mosquito to a pathogen is disclosed. The method comprising administering to a mosquito an isolated nucleic acid agent comprising a nucleic acid sequence which specifically downregulates an expression of at least one mosquito or pathogen gene wherein a product of said mosquito or pathogen gene participates in infection and/or growth of the pathogen in the mosquito.

Two or more conditional, dominant, lethal gene expression systems provide high levels of penetrance in insects. Lethality is induced at an earlier stage of development and the risk of biochemical resistance is reduced, as compared to a single insect conditional, dominant, lethal gene expression system. The invention is useful for the control of insect populations.

An all male Culicide mosquito population is created by knocking down its Transformer-2 gene, causing the dysfunction of X chromosome-bearing sperm, hence producing severe biased male progenies. Unlike previous methods, we recently discovered that the Tra-2 knockdown also results in female-specific zygotes lethality (XX). This art is therefore also designed to kill early female zygotes (XX) that may have survived the previous knockdown, and the all male progenies are created only when an antibiotic substance has been added into food and drink to feed mosquitoes. The strict limit of the antibiotic exposure time allows mosquito-adapted Wolbachia bacteria to survive. Selected Wolbachia bacteria may induce cytoplasmic incompatibility (CI) of up to 100%. All the progenies are therefore genetically males, which cause sterility when outcrossing with females infected by another Wolbachia strain (bidirectional CI) or are uninfected (unidirectional CI) in natural environment.

A composition-of-matter for mosquito control is provided. The composition comprises a cell which comprises an exogenous naked dsRNA which specifically down-regulates expression of a gene being endogenous to a mosquito or which specifically down-regulated expression of a gene being endogenous to a mosquito pathogen. Further provided is a composition-of-matter for mosquito control, comprising a cell comprising a nucleic acid larvicide. Also provided are methods of producing and using the compositions.