The present invention provides improved and/or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

Disclosed are improved methods for manufacturing large-scale populations of robust, highly pure, and functional T regulatory cells (Tregs). Also disclosed are expanded Treg populations, cryopreserved Treg populations and methods and uses of these cells in compositions formulated for treating one or more mammalian diseases, including, for example, treatment, prophylaxis, and/or amelioration of one or more symptoms of a human neurodegenerative disorder. In particular, the compositions and methods provided herein find clinical use in the treatment and amelioration of one or more symptoms of amyotrophic lateral sclerosis (ALS), Alzheimer's disease, and other neurological diseases and disorders.

The present invention discloses an in-vitro culture, induction, activation and cryopreservation method and cell bank establishment for immune cells. The method includes the follows: using a dedicated amplification medium of immune cells to perform first-stage amplification culture on mononuclear cells to obtain preliminarily amplified immune cells; using a dedicated induction medium of immune cells to perform second-stage induction and amplification culture on the preliminarily amplified immune cells to obtain induced immune cells; using a dedicated activation medium of immune cells to perform third-stage activation and amplification culture on the induced immune cells to obtain a large number of immune cells with activation functions; using a dedicated cryopreserving fluid of immune cells to cryopreserve the immune cells to obtain cryopreserved immune cells; and performing preservation according to ABO/RH typing and HLA typing; and establishing an information file of immune cells for retrieval to construct an immune cell bank.

The present invention relates to a shipping container for cryopreserved biological samples in which a cryopreserved sample can be maintained on arrival at its destination for a period of time, for example several months.

Disclosed is a device for the perfusion of an organ, including: a container of fluid, containing an organ bathed in the perfusion fluid; a first path including an inlet, an outlet and a pump; and a second path including an inlet, an outlet and a pump. The “arterial” outlet of the first path has a diameter smaller than a diameter of the “portal” outlet of the second path. The device additionally includes, between the pump and the outlet of the first path and/or between the pump and the outlet of the second path, an oxygenation unit arranged to oxygenate the fluid emerging from the “arterial” outlet of the first path more than the fluid emerging from the “portal” outlet of the second path. The device can include a communication path between the first path and the second path in order to oxygenate the second path. Use in liver transplantation.

Apparatus are provided for preventing the formation of ice crystals in a biological sample containing a non-Newtonian fluid as a cryopreservation medium. The apparatus may be used to prevent ice formation during cryopreservation of biological samples, or during warming of cryopreserved biological samples, by changing the viscosity of the non-Newtonian fluid. The apparatus (200) comprises a housing (202) for a container (212) containing the biological sample (214) and the non-Newtonian fluid, and a device (204) for inducing a change in viscosity of the cryopreservation medium. The change in viscosity may be increased by inducing shear thickening of the cryopreservation medium, or the change in viscosity may be decreased by inducing shear thinning of the cryopreservation medium. Possible viscosity-changing devices comprise a tapping device, a piston, a rotating device, a compression device, a sound generating device, a permanent magnet or a electromagnetic field generating device. The apparatus may further include a temperature control device.

Disclosed are devices and methods for non-cryogenic vitrification of biological materials that include the steps of providing one or more capillary channels of which a first opening is operably in contact with a moisture containing vitrification mixture made of a biological material and a vitrification agent.

According to the present invention, there are provided a chamber for transplantation which has a high durability, and in which an enclosed biological constituent can be maintained for a long period of time because an interior space thereof is efficiently secured; and a method for manufacturing the chamber for transplantation. The chamber for transplantation includes one or more membranes for immunoisolation at a boundary between an inside and an outside of the chamber for transplantation, in which all of the membranes for immunoisolation include a porous membrane containing a polymer, and a joint portion at which the porous membranes are directly fusion welded to each other is provided. The method for manufacturing a chamber for transplantation includes preparing one or more porous membranes containing a polymer selected from polysulfone and polyethersulfone, bringing one part of the porous membrane into direct contact with another part of the porous membrane, and performing a heat fusion welding of the two parts that are in direct contact with each other at a temperature which is a glass transition temperature of the polymer or higher and lower than a melting point of the polymer.

A system and method for maintaining the vitality of an organ through negative pressure ventilation and perfusion. The system includes fluidically coupled components: an organ enclosure, a diaphragm enclosure, an actuator/pump, a perfusion system, and a reservoir. The actuator can displace a precise amount of a working fluid that displaces that precise amount of a sterile support fluid. The sterile fluid travels between the diaphragm enclosure and the organ enclosure, thereby ventilating the organ within the organ chamber. The perfusion system circulates a perfusate through the organ.

The present invention relates to a method for freezing and preserving a composition comprising a population of neonatal stromal cells (NSCs) and a cryoprotector, characterised in that it comprises a step of freezing the composition at a temperature of between −70° C. and −140° C., then a step of preserving the composition at between −10° C. and −40° C. The present invention also relates to a composition comprising a population of NSCs and a cryoprotector, characterised in that it is preserved at between −10° C. and −40° C., said NSCs being in particular placental NSCs.