In a method for producing transparent biological preparations for examination by light microscopy biological tissue is electrophoretically clarified in that the tissue is immersed in an aqueous alkaline electrophoresis solution and is exposed to an electric field in the electrophoresis solution. The electrophoresis solution contains a buffer base, the cations of which have a molecular weight of at least 50 Da, at a concentration of 5 to 100 mol/m.sup.3 and a non-ionic detergent at a concentration of 0.1 to 10% (w/v).

A system and methodology for the preservation of red blood cells is described in which red blood cells are oxygen or oxygen and carbon dioxide depleted, treated and are stored in an anaerobic environment to optimize preparation for transfusion. More particularly, a system and method for extended storage of red blood cells from collection to transfusion that optimizes red blood cells prior to transfusion is described.

A method for preserving a tissue graft includes submerging the tissue graft in a solution containing one or more antimicrobial and/or stabilizing agents and storing the tissue graft in the solution for a period of time of at least 24 hours while the solution is in an unfrozen state. A tissue preservation system includes a solution containing one or more antimicrobial and/or stabilizing agents and a tissue graft in the solution.

A system and methodology for the preservation of red blood cells is described in which red blood cells are oxygen or oxygen and carbon dioxide depleted, treated and are stored in an anaerobic environment to optimize preparation for transfusion. More particularly, a system and method for extended storage of red blood cells from collection to transfusion that optimizes red blood cells prior to transfusion is described.

Embodiments include exposing blood to a pulsed magnetic field. Embodiments include exposing blood to a pulsed magnetic field as a means of improving blood viscosity management, blood oxygenation, and banked blood shelf life. Various other embodiments are also included herein.

One aspect of the invention provides a method for preparing a vein graft. The method includes: applying a tissue passivation agent to a resected anatomical vessel; placing the resected anatomical vessel in a chamber; and allowing the tissue passivation agent to cross-link while the resected anatomical vessel is in the chamber.

This disclosure describes compositions and methods related to cryoprotection of biomaterial. Generally, the cryoprotective composition includes a cryoprotective agent and magnetic nanoparticles effective for thawing a cryopreserved specimen comprising biomaterial with minimal biomaterial damage. In some embodiments, the composition is effective for thawing a cryopreserved specimen having a minimum dimension of 0.1 mm. Generally, the method includes obtaining a biomaterial cryopreserved with a cryoprotective composition as summarized above, then subjecting the cryopreserved biomaterial to electromagnetic energy of an intensity sufficient to excite the magnetic nanoparticles and thaw the biomaterial.

A cryopreservation composition generally includes a cryoprotective agent and a laser absorber that includes a metal. Rewarming a cryopreserved specimen that includes the cryopreservation composition includes subjecting the cryopreserved specimen to a laser pulse effective to heat the laser absorber sufficiently to thaw the cryopreserved biospecimen.

Provided herein are systems and methods for implementing a modular light device for use in an electronic treatment device according to examples of the disclosure. In one or more examples, the modular light device can be implemented as a stand-alone component that can be swapped in and out of the treatment device. In one or more examples, the light device can include a light source array chamber configured to transmit UV light of a selected wavelength, the light source array chamber including one or more light source arrays and light sensors collectively configured as part of the light device to deliver light to a biological sample.

The present invention relates to a method of freezing of biological material and a freezing apparatus for freezing of biological material.