A sperm extender medium for prolonging the fertility of bovine spermatozoa during liquid storage of the spermatozoa. The medium includes a monosaccharide energy source for the spermatozoa. The one or more or amino acid osmolytes are selected from mono-amino carboxylic and mono-amino sulfonic acids, each independently having an R side group selected from the group consisting of H and non-polar, neutral open aliphatic chains excluding branched C3 and longer aliphatic chains. The medium also includes one or more antioxidants and physiologically acceptable inorganic salts for maintaining sperm viability, and is buffered for maintaining pH in a range of from about 7.0 to about 8.5. Further, the medium may have a concentration of sodium ions of less than about 95 mM, and an osmolarity in a range of from about 290 mOsm/L to about 400 mOsm/L. Preparations including the spermatozoa and methods for preparing the medium and methods for fertilisation of an ovum.

Provided are systems and methods for treating a biological fluid, e.g., to inactivate pathogens.

The present invention provides pathogen-inactivated red blood cell compositions suitable for infusion into a subject.

The present invention provides pathogen-inactivated red blood cell compositions suitable for infusion into a subject.

The present invention is directed to methods for collecting and processing autografts, processed autografts, kits for collecting and transporting autografts, and tools for preparing autografts. It is also directed to autologous bone grafts, and methods of preparing them.

A perfusion loop assembly for ex vivo liver perfusion includes a pump providing perfusion fluid through a line branching at a branching point into a first branch line and a second branch line. The first branch line provides a first portion of the perfusion fluid to the hepatic artery of the liver, the first branch line coupled with a gas exchanger, where the first branch line includes a flow rate sensor and/or a pressure sensor. The second branch line provides a second portion of the perfusion fluid to the portal vein of the liver; the second branch line includes a valve for controlling flow of perfusion fluid into the portal vein. The second branch line includes a flow rate sensor and/or a pressure sensor. A liver chamber assembly holds the liver ex vivo, and an outlet line for the perfusion fluid connects the liver chamber assembly and the pump.

A perfusion loop assembly for ex vivo liver perfusion includes a pump providing perfusion fluid through a line branching at a branching point into a first branch line and a second branch line. The first branch line provides a first portion of the perfusion fluid to the hepatic artery of the liver, the first branch line coupled with a gas exchanger, where the first branch line includes a flow rate sensor and/or a pressure sensor. The second branch line provides a second portion of the perfusion fluid to the portal vein of the liver; the second branch line includes a valve for controlling flow of perfusion fluid into the portal vein. The second branch line includes a flow rate sensor and/or a pressure sensor. A liver chamber assembly holds the liver ex vivo, and an outlet line for the perfusion fluid connects the liver chamber assembly and the pump.

Described herein are hydrogel-based nanoparticles which release nitric oxide (NO) or other bioactive forms of NO including nitrosothiols, nitrofatty acids and dinitrogen trioxide into stored red blood cells (RBCs). Also provided herein is a method for using hydrogel-based nanoparticles to supplement stored RBCs with NO to enhance red blood cell (RBC) storage time, improve survivability in circulation, minimize toxicity associated with transfusion, and improve transfusion safety by eliminating infective organisms in stored blood.

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes an antimicrobial treatment mechanism to inactivate microbes flushed from the biological sample by preservation fluid flowed therethrough. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature while simultaneously treating microbial infections to prevent transmission between a donor and a recipient.

The present invention provides an acellular collagenous tissue and a method for processing an artificial heart valve containing the acellular collagenous tissue. The method comprises immersing the acellular collagenous tissue in a preservation liquid containing an ionic liquid until the size of the acellular collagenous tissue remains stable in a non-liquid environment. The present invention also provides a preservation method, comprising immersing the acellular collagenous tissue or the artificial heart valve containing acellular collagenous tissue in the preservation liquid containing ionic liquid until the size of the collagen tissue remains stable in the non-liquid environment. The present invention also provides an acellular collagenous tissue and an artificial heart valve containing the acellular collagenous tissue processed by the method described above. The method can effectively avoid a biological material damage caused by being in a non-liquid environment and is beneficial to implement a subsequent processing and sterilization for the biological material.